Recombinant Human TOP1 Protein (Met1-Phe765), His-tagged

• Cat.No.: TOP1-664H
• Product Overview: Recombinant Human TOP1 Protein (NP_003277.1) (Met1-Phe765) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with a polyhistidine tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile 20 mM Tris, pH 7.5, 200 mM NaCl, 20 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human TOP1 consists of 784 amino acids and predicts a molecular mass of 93.1 kDa.
• Protein length: Met1-Phe765
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: TOP1 topoisomerase (DNA) I [ Homo sapiens ]
• Official Symbol: TOP1
• Synonyms: TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; TOPI
• Gene ID: 7150
• mRNA Refseq: NM_003286
• Protein Refseq: NP_003277
• MIM: 126420
• UniProt ID: P11387