Cat.:	TOP1-664H
Cat.No.:	TOP1-664H
Product Overview:	Recombinant Human TOP1 Protein (NP_003277.1) (Met1-Phe765) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with a polyhistidine tag at the N-terminus.
Source:	Baculovirus-Insect Cells
Species:	Human
Tag :	His
Predicted N Terminal:	His
Form:	Lyophilized from sterile 20 mM Tris, pH 7.5, 200 mM NaCl, 20 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass:	The recombinant human TOP1 consists of 784 amino acids and predicts a molecular mass of 93.1 kDa.
Protein length:	Met1-Phe765
Endotoxin:	< 1.0 EU per μg protein as determined by the LAL method.
Purity:	> 95 % as determined by SDS-PAGE.
Stability:	Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage:	Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping:	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Name:	TOP1 topoisomerase (DNA) I [ Homo sapiens ]
Official Symbol:	TOP1
Synonyms:	TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; TOPI;
Gene ID:	7150
mRNA Refseq:	NM_003286
Protein Refseq:	NP_003277
MIM:	126420
UniProt ID:	P11387

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