Recombinant Human LIPE, His-tagged

• Cat.No.: LIPE-26698TH
• Product Overview: Recombinant fragment, corresponding to amino acids 597-1076 of Human HSL/LIPE with N terminal His tag; 480 amino acids, 59kDa.

Specification

• Description: The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 95 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Store at 4°C. Upon reconstitution store at -80oC.
• Sequences of amino acids: GPGPVLVRLISYDLREGQDSEELSS LIKSNGQRSLELWPR PQQAPRSRSLIVHFHGGGFVAQTSR SHEPYLKSWAQEL GAPIISIDYSLAPEAPFPRALEECF FAYCWAIKHCALLGS TGERICLAGDSAGGNLCFTVALRAA AYGVRVPDGIMAA YPATMLQPAASPSRLLSLMDPLLPL SVLSKCVSAYAGA KTEDHSNSDQKALGMMGLVRRDTAL LLRDFRLGASSWLNS FLELSGRKSQKMSEPIAEPMRRSVS EAALAQPQGPLGT DSLKNLTLRDLSLRGNSETSSDTPE MSLSAETLSPSTP SDVNFLLPPEDAGEEAEAKNELSPM DRGLGVRAAFPEGFH PRRSSQGATQMPLYSSPIVKNPFMS PLLAPDSMLKSLP PVHIVACALDPMLDDSVMLARRLRN LGQPVTLRVVEDLPHGFLTLAALCR ETRQAAELCVERIRLVLTPPAGAGP SGE TGAAGVDGGCGGRH
• Sequence Similarities: Belongs to the GDXG lipolytic enzyme family.

Gene Information

• Gene Name: LIPE lipase, hormone-sensitive [ Homo sapiens ]
• Official Symbol: LIPE
• Synonyms: LIPE; lipase, hormone-sensitive; hormone-sensitive lipase; HSL
• Gene ID: 3991
• mRNA Refseq: NM_005357
• Protein Refseq: NP_005348
• MIM: 151750
• Uniprot ID: Q05469
• Chromosome Location: 19q13.1-q13.2
• Pathway: Adipogenesis, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem
• Function: acylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; triglyceride lipase activity