Recombinant Human Adenine phosphoribosyltransferase

• Cat.No.: APRT-1939H
• Product Overview: Recombinant human APRT protein was expressed inE.coliand purified by using conventional chromatography techniques. 19.6 kDa (180aa).

Specification

• Cat. No.: APRT-1939H
• Description: APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway.
• Source: E.coli.
• Purity: > 90% by SDS – PAGE.
• Physical Appearance: Liquid.
• Formulation: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLAR HLKATHGG RIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRGKLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: APRT adenine phosphoribosyltransferase [ Homo sapiens ]
• Synonyms: APRT; adenine phosphoribosyltransferase; AMP; MGC125856; MGC125857; MGC129961; DKFZp686D13177; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase; EC 2.4.2.7
• Gene ID: 353
• mRNA Refseq: NM_000485
• Protein Refseq: NP_000476
• MIM: 102600
• UniProt ID: P07741
• Chromosome Location: 16q24
• Pathway: Metabolic pathways; Purine metabolism; Metabolism of nucleotides
• Function: AMP binding; adenine binding; adenine phosphoribosyltransferase activity; protein binding; transferase activity, transferring glycosyl groups