||Recombinant human APRT protein was expressed inE.coliand purified by using conventional chromatography techniques. 19.6 kDa (180aa).
||APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway.
||> 90% by SDS – PAGE.
||In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLAR HLKATHGG RIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRGKLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.