|Product Overview:||Recombinant Human DKK1, Thr32-His266, was produced in Sf 21 (baculovirus)-derived under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.|
|Molecular Mass:||Predicted Molecular Mass: 25.8 kDa
SDS-PAGE: 33-38 kDa, reducing conditions
|N-terminal Sequence Analysis:||Thr-Leu-Asn-Ser-Val-Leu-Asn-Ser-Asn-Ala|
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]|
|Synonyms:||DKK1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1;|