Recombinant Full Length Human GLS Protein, C-Flag-tagged

• Cat.No.: GLS-270HFL
• Product Overview: Recombinant Full Length Human GLS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 73.3 kDa
• AA Sequence: MMRLRGSGMLRDLLLRSPAGVSATL RRAQPLVTLCRRPRGGGRPAAGPAA AARLHPWWGGGGWPAEPLAR GLSSSPSEILQELGKGSTHPQPGVS PPAAPAAPGPKDGPGETDAFGNSEG KELVASGENKIKQGLLPSLE DLLFYTIAEGQEKIPVHKFITALKS TGLRTSDPRLKECMDMLRLTLQTTS DGVMLDKDLFKKCVQSNIVL LTQAFRRKFVIPDFMSFTSHIDELY ESAKKQSGGKVADYIPQLAKFSPDL WGVSVCTVDGQRHSTGDTKV PFCLQSCVKPLKYAIAVNDLGTEYV HRYVGKEPSGLRFNKLFLNEDDKPH NPMVNAGAIVVTSLIKQGVN NAEKFDYVMQFLNKMAGNEYVGFSN ATFQSERESGDRNFAIGYYLKEKKC FPEGTDMVGILDFYFQLCSI EVTCESASVMAATLANGGFCPITGE RVLSPEAVRNTLSLMHSCGMYDFSG QFAFHVGLPAKSGVAGGILL VVPNVMGMMCWSPPLDKMGNSVKGI HFCHDLVSLCNFHNYDNLRHFAKKL DPRREGGDQRVKSVINLLFA AYTGDVSALRRFALSAMDMEQRDYD SRTALHVAAAEGHVEVVKFLLEACK VNPFPKDRWNNTPMDEALHF
  GHHDVFKILQEYQVQYTPQGDSDNG KENQTVHKNLDGLLSGPTRTRRLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

Gene Information

• Gene Name: GLS glutaminase [ Homo sapiens (human) ]
• Official Symbol: GLS
• Synonyms: GAC; GAM; KGA; GLS1; AAD20; DEE71; GDPAG; CASGID; EIEE71
• Gene ID: 2744
• mRNA Refseq: NM_014905.5
• Protein Refseq: NP_055720.3
• MIM: 138280
• UniProt ID: O94925