Active Recombinant Human GLS Protein, His-tagged

• Cat.No.: GLS-08H
• Product Overview: Recombinant human Glutaminase protein (Ser17-Leu669) with N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to hydrolyze glutamine to glutamate. The specific activity is >12000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 73 kDa
• AA Sequence: MAGHLASDFAFSPPPGGGGDGPGGP EPGWVDPRTWLSFQGPPGGPGIGPG VGPGSEVWGIPPCPPPYEFCGGMAY CGPQVGVGLVPQGGLETSQPEGEAG VGVESNSDGASPEPCTVTPGAVKLE KEKLEQNPEESQDIKALQKELEQFA KLLKQKRITLGYTQADVGLTLGVLF GKVFSQTTICRFEALQLSFKNMCKL RPLLQKWVEEADNNENLQEICKAET LVQARKRKRTSIENRVRGNLENLFL QCPKPTLQQISHIAQQLGLEKDVVR VWFCNRRQKGKRSSSDYAQREDFEA AGSPFSGGPVSFPLAPGPHFGTPGY GSPHFTALYSSVPFPEGEAFPPVSV TTLGSPMHSNESGGGGSPGRRRRRR RRRRR
• N-terminal Sequence Analysis: Met
• Purity: > 80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Enzyme Activity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP, Glycerol and CHAPS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: GLS glutaminase [ Homo sapiens (human) ]
• Official Symbol: GLS
• Synonyms: GLS; glutaminase; GAC; GAM; KGA; GLS1; AAD20; DEE71; GDPAG; CASGID; EIEE71; glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase; glutaminase C; glutaminase, phosphate-activated; EC 3.5.1.2
• Gene ID: 2744
• mRNA Refseq: NM_014905
• Protein Refseq: NP_055720
• MIM: 138280
• UniProt ID: O94925