Recombinant Human ALK Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALK-1272H
• Product Overview: ALK MS Standard C13 and N15-labeled recombinant protein (NP_004295) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 176.3 kDa
• AA Sequence: MGAIGLLWLLPLLLSTAAVGSGMGT GQRAGSPAAGPPLQPREPLSYSRLQ RKSLAVDFVVPSLFRVYARDLLLPP SSSELKAGRPEARGSLALDCAPLLR LLGPAPGVSWTAGSPAPAEARTLSR VLKGGSVRKLRRAKQLVLELGEEAI LEGCVGPPGEAAVGLLQFNLSELFS WWIRQGEGRLRIRLMPEKKASEVGR EGRLSAAIRASQPRLLFQIFGTGHS SLESPTNMPSPSPDYFTWNLTWIMK DSFPFLSHRSRYGLECSFDFPCELE YSPPLHDLRNQSWSWRRIPSEEASQ MDLLDGPGAERSKEMPRGSFLLLNT SADSKHTILSPWMRSSSEHCTLAVS VHRHLQPSGRYIAQLLPHNEAAREI LLMPTPGKHGWTVLQGRIGRPDNPF RVALEYISSGNRSLSAVDFFALKNC SEGTSPGSKMALQSSFTCWNGTVLQ LGQACDFHQDCAQGEDESQMCRKLP VGFYCNFEDGFCGWTQGTLSPHTPQ WQVRTLKDARFQDHQDHALLLSTTD VPASESATVTSATFPAPIKSSPCEL RMSWLIRGVLRGNVSLVLVENKTGK EQGRMVWHVAAYEGLSLWQWMVLPL LDVSDRFWLQMVAWWGQGSRAIVAF DNISISLDCYLTISGEDKILQNTAP KSRNLFERNPNKELKPGENSPRQTP IFDPTVHWLFTTCGASGPHGPTQAQ CNNAYQNSNLSVEVGSEGPLKGIQI WKVPATDTYSISGYGAAGGKGGKNT MMRSHGVSVLGIFNLEKDDMLYILV GQQGEDACPSTNQLIQKVCIGENNV IEEEIRVNRSVHEWAGGGGGGGGAT YVFKMKDGVPVPLIIAAGGGGRAYG AKTDTFHPERLENNSSVLGLNGNSG AAGGGGGWNDNTSLLWAGKSLQEGA TGGHSCPQAMKKWGWETRGGFGGGG GGCSSGGGGGGYIGGNAASNNDPEM DGEDGVSFISPLGILYTPALKVMEG HGEVNIKHYLNCSHCEVDECHMDPE SHKVICFCDHGTVLAEDGVSCIVSP TPEPHLPLSLILSVVTSALVAALVL AFSGIMIVYRRKHQELQAMQMELQS PEYKLSKLRTSTIMTDYNPNYCFAG KTSSISDLKEVPRKNITLIRGLGHG AFGEVYEGQVSGMPNDPSPLQVAVK TLPEVCSEQDELDFLMEALIISKFN HQNIVRCIGVSLQSLPRFILLELMA GGDLKSFLRETRPRPSQPSSLAMLD LLHVARDIACGCQYLEENHFIHRDI AARNCLLTCPGPGRVAKIGDFGMAR DIYRASYYRKGGCAMLPVKWMPPEA FMEGIFTSKTDTWSFGVLLWEIFSL GYMPYPSKSNQEVLEFVTSGGRMDP PKNCPGPVYRIMTQCWQHQPEDRPN FAIILERIEYCTQDPDVINTALPIE YGPLVEEEEKVPVRPKDPEGVPPLL VSQQAKREEERSPAAPPPLPTTSSG KAAKKPTAAEVSVRVPRGPAVEGGH VNMAFSQSNPPSELHRVHGSRNKPT SLWNPTYGSWFTEKPTKKNNPIAKK EPHERGNLGLEGSCTVPPNVATGRL PGASLLLEPSSLTANMKEVPLFRLR HFPCGNVNYGYQQQGLPLEAATAPG AGHYEDTILKSKNSMNQPGPTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALK anaplastic lymphoma receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; anaplastic lymphoma receptor tyrosine kinase; anaplastic lymphoma kinase (Ki 1); ALK tyrosine kinase receptor; CD246; CD246 antigen; mutant anaplastic lymphoma kinase; NBLST3
• Gene ID: 238
• mRNA Refseq: NM_004304
• Protein Refseq: NP_004295
• MIM: 105590
• UniProt ID: Q9UM73