Active Recombinant Human HGF protein

• Cat.No.: HGF-195H
• Product Overview: Recombinant Human HGF( HGF protein isoform 1 (comprising the signal peptide, and alpha and beta chains)) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Form: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Bio-activity: The ED50 of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.
• Molecular Mass: Under reducing conditions HGF migrates as a broad band between 80 and 110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified HGF that has a predicted monomeric molecular mass of 79.7 kDa.
• Purity: >95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue.
• Storage: Lyophilised products should be stored at 2 to 8 centigrade. Following reconstitution short-term storage at 4 centigrade is recommended and longer-term storage of aliquots at -18 to -20 centigrade.
  Repeated freeze thawing is not recommended.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Official Symbol: HGF
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39 , DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210