| Description : |
This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015] |
| Source : |
Insects cell |
| Species : |
Human |
| Tag : |
Tag Free |
| Form : |
Powder |
| Bio-activity : |
Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/mL. |
| AA Sequence : |
Alpha chain: QRKRRNTIHEFKKSAKTTLIKIDPA LKIKTKKVNTADQCANRCTRNKGLP FTCKAFVFDKARKQCLWFPFNSMSS GVKKEFGHEFDLYENKDYIRNCIIG KGRSYKGTVSITKSGIKCQPWSSMI PHEHSFLPSSYRGKDLQENYCRNPR GEEGGPWCFTSNPEVRYEVCDIPQC SEVECMTCNGESYRGLMDHTESGKI CQRWDHQTPHRHKFLPERYPDKGFD DNYCRNPDGQPRPWCYTLDPHTRWE YCAIKTCADNTMNDTDVPLETTECI QGQGEGYRGTVNTIWNGIPCQRWDS QYPHEHDMTPENFKCKDLRENYCRN PDGSESPWCFTTDPNIRVGYCSQIP NCDMSHGQDCYRGNGKNYMGNLSQT RSGLTCSMWDKNMEDLHRHIFWEPD ASKLNENYCRNPDDDAHGPWCYTGN PLIPWDYCPISRCEGDTTPTIVNLD HPVISCAKTKQLR
Beta chain: VVNGIPTRTNIGWMVSLRYRNKHIC GGSLIKESWVLTARQCFPSRDLKDY EAWLGIHDVHGRGDEKCKQVLNVSQ LVYGPEGSDLVLMKLARPAVLDDFV STIDLPNYGCTIPEKTSCSVYGWGY TGLINYDGLLRVAHLYIMGNEKCSQ HHRGKVTLNESEICAGAEKIGSGPC EGDYGGPLVCEQHKMRMVLGVIVPG RGCAIPNRPGIFVRVAYYAKWIHKI ILTYKVPQS |
| Endotoxin : |
Endotoxin level is < 0.1 ng/μg of the protein (< 1 EU/μg). |
| Purity : |
> 98% by SDS-PAGE gel and HPLC analyses. |
| Applications : |
SDS-PAGE |
| Notes : |
For laboratory research only, not for drug, diagnostic or other use. |
| Storage : |
Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Storage Buffer : |
10 mM Tris (pH 8.0), 100 mM L-Arginine HCl and 200 mM NaCl. |
| Reconstitution : |
Initially reconstitute in water to 0.5 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. |
