Recombinant Human KDM4B(1-363) Protein, N-His-tagged

• Cat.No.: KDM4B-118H
• Product Overview: N-terminal 6Histagged, recombinant, amino acids 1-363, human JMJD2B, expressed in E.coli. Purified using immobilised metal affinity chromatography.

Specification

• Description: JMJD2B (Jumonji domain-containing protein 2B), also known as KDM4B, is a histone demethylase that belongs to the JmjC domain-containing protein family. It specifically targets di- and tri-methylated lysine residues on histones H3 and H4, and catalyzes their removal, leading to changes in chromatin structure and gene expression. JMJD2B is expressed in a variety of tissues, including the brain, heart, and liver, and is involved in the regulation of various cellular processes, including differentiation, proliferation, and apoptosis. JMJD2B has been shown to play a role in the development of several types of cancer, including breast, prostate, and lung cancers. It has also been implicated in the regulation of various viral infections, including human immunodeficiency virus (HIV) and human papillomavirus (HPV). Therefore, JMJD2B has emerged as a potential target for the development of novel therapeutics for cancer and viral infections.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: 1-363 aa
• Form: 0.8mg/ml of enzyme in 25mM Tris/HCl pH 7.5, 250mM NaCl, 50% glycerol. Frozen solution.
• Molecular Mass: 44.3 kDa
• AA Sequence: 1 MHHHHHHSSG VDLGTENLYF QSMGSEDHGA QNPSCKIMTF RPTMEEFKDF 51 NKYVAYIESQ GAHRAGLAKI IPPKEWKPRQ TYDDIDDVVI PAPIQQVVTG 101 QSGLFTQYNI QKKAMTVGEY RRLANSEKYC TPRHQDFDDL ERKYWKNLTF 151 VSPIYGADIS GSLYDDDVAQ WNIGSLRTIL DMVERECGTI IEGVNTPYLY 201 FGMWKTTFAW HTEDMDLYSI NYLHFGEPKS WYAIPPEHGK RLERLAIGFF 251 PGSSQGCDAF LRHKMTLISP IILKKYGIPF SRITQEAGEF MITFPYGYHA 301 GFNHGFNCAE STNFATLRWI DYGKVATQCT CRKDMVKISM DVFVRILQPE 351 RYELWKQGKD LTVLDHTRPT ALTSPELSSW SASRA
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: Stable for 1 year at -70 centigrade from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: KDM4B lysine demethylase 4B [ Homo sapiens (human) ]
• Official Symbol: KDM4B
• Synonyms: KDM4B; lysine demethylase 4B; MRD65; JMJD2B; TDRD14B
• Gene ID: 23030
• mRNA Refseq: NM_015015.3
• Protein Refseq: NP_055830.1
• MIM: 609765
• UniProt ID: O94953