Recombinant Human KIT Protein, His-tagged

• Cat.No.: KIT-121H
• Product Overview: Recombinant human KIT protein with His tag was expressed in HEK293.

Specification

• Description: This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 57.1 kDa
• Protein Length: 976
• AA Sequence: MRGARGAWDFLCVLLLLLRVQTGSS QPSVSPGEPSPPSIHPGKSDLIVRV GDEIRLLCTDPGFVKWTFEILDETN ENKQNEWITEKAEATNTGKYTCTNK HGLSNSIYVFVRDPAKLFLVDRSLY GKEDNDTLVRCPLTDPEVTNYSLKG CQGKPLPKDLRFIPDPKAGIMIKSV KRAYHRLCLHCSVDQEGKSVLSEKF ILKVRPAFKAVPVVSVSKASYLLRE GEEFTVTCTIKDVSSSVYSTWKREN SQTKLQEKYNSWHHGDFNYERQATL TISSARVNDSGVFMCYANNTFGSAN VTTTLEVVDKGFINIFPMINTTVFV NDGENVDLIVEYEAFPKPEHQQWIY MNRTFTDKWEDYPKSENESNIRYVS ELHLTRLKGTEGGTYTFLVSNSDVN AAIAFNVYVNTKPEILTYDRLVNGM LQCVAAGFPEPTIDWYFCPGTEQRC SASVLPVDVQTLNSSGPPFGKLVVQ SSIDSSAFKHNGTVECKAYNDVGKT SAYFNFAFKGNNKEQIHPHTLFTPL LIGFVIVAGMMCIIVMILTYKYLQK PMYEVQWKVVEEINGNNYVYIDPTQ LPYDHKWEFPRNRLSFGKTLGAGAF GKVVEATAYGLIKSDAAMTVAVKML KPSAHLTEREALMSELKVLSYLGNH MNIVNLLGACTIGGPTLVITEYCCY GDLLNFLRRKRDSFICSKQEDHAEA ALYKNLLHSKESSCSDSTNEYMDMK PGVSYVVPTKADKRRSVRIGSYIER DVTPAIMEDDELALDLEDLLSFSYQ VAKGMAFLASKNCIHRDLAARNILL THGRITKICDFGLARDIKNDSNYVV KGNARLPVKWMAPESIFNCVYTFES DVWSYGIFLWELFSLGSSPYPGMPV DSKFYKMIKEGFRMLSPEHAPAEMY DIMKTCWDADPLKRPTFKQIVQLIE KQISESTNHIYSNLANCSPNRQKPV VDHSVRINSVGSTASSSQPLLVHDD V
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens (human) ]
• Official Symbol: KIT
• Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit
• Gene ID: 3815
• mRNA Refseq: NM_000222
• Protein Refseq: NP_000213
• MIM: 164920
• UniProt ID: P10721