Recombinant Mouse KIT protein(Met1-Thr523), His-tagged

• Cat.No.: KIT-1138M
• Product Overview: Recombinant Mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Thr523
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse KIT at 2 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear range of 1.28-6.4 ng/ml.
• Molecular Mass: The secreted recombinant mouse KIT comprises 510 amino acids and has a predicted molecular mass of 57 kDa. As a result of glycosylation, the apparent molecular mass of rmKIT is approximately 70-80 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Kit kit oncogene [ Mus musculus ]
• Official Symbol: KIT
• Synonyms: KIT; kit oncogene; mast/stem cell growth factor receptor Kit; SCFR; belly-spot; dominant spotting; proto-oncogene c-Kit; spotted sterile male; Steel Factor Receptor; Dominant white spotting; tyrosine-protein kinase Kit; c-kit proto-oncogene protein; proto-oncogene tyrosine-protein kinase Kit; W; Bs; Fdc; Ssm; SCO1; SCO5; SOW3; CD117; c-KIT; Tr-kit; Gsfsco1; Gsfsco5; Gsfsow3
• Gene ID: 16590
• mRNA Refseq: NM_001122733
• Protein Refseq: NP_001116205