Recombinant Human MGMT Protein (M1-N207), His/Strep tagged

• Cat.No.: MGMT-0598H
• Product Overview: Recombinant Human 6His-strepII-TEV-GG-MGMT(M1-N207 end) Protein was expressed in E. coli.

Specification

• Description: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
• Source: E. coli
• Species: Human
• Tag: His/Strep
• Form: Liquid
• Protein Length: M1-N207
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens (human) ]
• Official Symbol: MGMT
• Synonyms: MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• Gene ID: 4255
• mRNA Refseq: NM_002412
• Protein Refseq: NP_002403
• MIM: 156569
• UniProt ID: P16455