Recombinant Human MGMT Protein, GST-tagged

• Cat.No.: MGMT-5304H
• Product Overview: Human MGMT full-length ORF ( AAH00824, 1 a.a. - 207 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 48.4 kDa
• AA Sequence: MDKDCEMKRTTLDSPLGKLELSGCE QGLHEIKLLGKGTSAADAVEVPAPA AVLGGPEPLMQCTAWLNAYFHQPEA IEEFPVPALHHPVFQQESFTRQVLW KLLKVVKFGEVISYQQLAALAGNPK AARAVGGAMRGNPVPILIPCHRVVC SSGAVGNYSGGLAVKEWLLAHEGHR LGKPGLGGSSGLAGAWLKGAGATSG SPPAGRN
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
• Official Symbol: MGMT
• Synonyms: MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
• Gene ID: 4255
• mRNA Refseq: NM_002412
• Protein Refseq: NP_002403
• MIM: 156569
• UniProt ID: P16455

Reviews

05/31/2021

The MGMT protein is an exceptional protein solution that exceeds expectations in terms of quality and suitability for my experimental requirements.

12/15/2018

In addition to its outstanding quality, the MGMT protein comes with exceptional technical support from its manufacturer.

03/07/2017

I am confident that utilizing the MGMT protein will empower me to uncover crucial insights into cellular mechanisms with precision.

Q&As

Can MGMT status be used to predict the response to other types of cancer treatments?

Yes, MGMT status has implications beyond glioblastoma and can influence the response to various alkylating agents in different cancer types.

Can the MGMT protein be targeted for cancer prevention strategies?

While direct targeting is challenging, understanding MGMT's role may lead to the development of preventive strategies, such as identifying high-risk individuals for regular screenings.

Is MGMT involved in resistance mechanisms to chemotherapy?

Yes, MGMT expression can confer resistance to alkylating agents, making it an important factor in understanding and overcoming chemotherapy resistance.

How does MGMT repair DNA damage caused by environmental factors like radiation?

MGMT repairs DNA damage by directly removing alkyl groups from the O6-position of guanine, which can be induced by exposure to radiation and certain chemicals.

How does MGMT status affect the choice of chemotherapy in glioblastoma patients?

Glioblastoma patients with MGMT promoter methylation are more likely to benefit from alkylating agents like temozolomide.