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Recombinant Human SIRPA Protein, MIgG1 Fc-tagged

Cat.No.: SIRPA-346H
Product Overview: Recombinant Human SIRPA Protein, MIgG1 Fc-tagged is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). Predicted N-terminus: Glu 31. This protein carries a mouse IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.
Source: HEK293
Species: Human
Tag : MIgG1 Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Human CD47, His Tag at 2 μg/mL can bind Recombinant Human SIRPA Protein, MIgG1 Fc-tagged with a linear range of 8-250 ng/mL (QC tested).
FACS assay shows that Recombinant Human SIRPA Protein, MIgG1 Fc-tagged can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 ug/ml (Routinely tested).
FACS analysis shows that the binding of Recombinant Human SIRPA Protein, MIgG1 Fc-tagged to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 ug/ml. IC50=0.1318 ug/ml (Routinely tested).
Molecular Mass: 63.5 kDa
Protein length: Glu 31 - Arg 370
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
Official Symbol: SIRPA
Synonyms: SIRPA; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; MYD-1; CD172A; PTPNS1;
Gene ID: 140885
mRNA Refseq: NM_001040022
Protein Refseq: NP_001035111
MIM: 602461
UniProt ID: P78324

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