Unactive Recombinant Human MAPKAPK3(2-end) Protein, N-GST-tagged

• Cat.No.: MAPKAPK3-134H
• Product Overview: N-terminal, GSTtagged, recombinant human MAPKAP Kinase 3, amino acids 2–end, expressed in E.coli cells. Purified using glutathione agarose.

Specification

• Description: MAPKAP Kinase 3 is a stress-activated serine/threonine-protein kinase which involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 2-end
• Form: 3.177mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with SAPK2a.
• Molecular Mass: 69.8 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SDGETAEEQG 241 GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL GLGVNGKVLE CFHRRTGQKC 301 ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI LDVYENMHHG KRCLLIIMEC MEGGELFSRI 361 QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF 421 AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI 481 SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN HPWINQSMVV 541 PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK IKDLKTSNNR LLNKRRKKQA 601 GSSSASQGCN NQ
• Purity: Purity 81.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAPKAPK3 MAPK activated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK3
• Synonyms: MAPKAPK3; MAPK activated protein kinase 3; 3PK; MK3; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Gene ID: 7867
• mRNA Refseq: NM_004635.5
• Protein Refseq: NP_004626.1
• MIM: 602130
• UniProt ID: Q16644