Active Recombinant Cynomolgus FCGR2A protein, His-tagged

• Cat.No.: FCGR2A-1472C
• Product Overview: Recombinant Cynomolgus FCGR2A protein(Gln28-Pro208), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Cynomolgus CD32a, His Tag on HIS1K Biosensor, can bind Rituximab with an affinity constant of 3.3 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Rituximab on FAB2G Biosensor, can bind Cynomolgus CD32a, His Tag with an affinity constant of 6.0 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 22.1 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln28-Pro208
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.