| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Cynomolgus Mesothelin (296-580), His Tag at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL.Immobilized Cynomolgus Mesothelin (296-580), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag with a linear range of 2-39 ng/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 33.7 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Asp296-Gly580 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Official Symbol : |
MSLN |
