Active Recombinant Full Length Human BTK Protein, N-His-tagged

• Cat.No.: BTK-31HFL
• Product Overview: N-terminal 6Histagged, recombinant, full-length, human BTK, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: BTK (Bruton's Tyrosine Kinase) is a member of the Tec family kinases which are important components of both antigen receptor signaling and other cell surface receptors, resulting in regulation of the actin cytoskeleton, adhesion, migration, and transcriptional activation. BTK is found in all cells of the hematopoietic lineage except plasma and T cells, and is required for normal B cell development and signal transduction through cell surface molecules. Mutations in the btk gene result in aberrant B cell development, leading to the X-linked agammaglobulinemia phenotype in humans and to the less severe X-linked immunodeficiency in mice. BTK inhibitors may be of benefit in the treatment of leukemias and lymphomas.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.31mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 521U/mg, where one unit of BTK, active activity is defined as 1nmol phosphate incorporated into 250μM cdc2 peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78.4 kDa
• AA Sequence: 1 MHHHHHHEFK GLRRRMAAVI LESIFLKRSQ QKKKTSPLNF KKRLFLLTVH KLSYYEYDFE 61 RGRRGSKKGS IDVEKITCVE TVVPEKNPPP ERQIPRRGEE SSEMEQISII ERFPYPFQVV 121 YDEGPLYVFS PTEELRKRWI HQLKNVIRYN SDLVQKYHPC FWIDGQYLCC SQTAKNAMGC 181 QILENRNGSL KPGSSHRKTK KPLPPTPEED QILKKPLPPE PAAAPVSTSE LKKVVALYDY 241 MPMNANDLQL RKGDEYFILE ESNLPWWRAR DKNGQEGYIP SNYVTEAEDS IEMYEWYSKH 301 MTRSQAEQLL KQEGKEGGFI VRDSSKAGKY TVSVFAKSTG DPQGVIRHYV VCSTPQSQYY 361 LAEKHLFSTI PELINYHQHN SAGLISRLKY PVSQQNKNAP STAGLGYGSW EIDPKDLTFL 421 KELGTGQFGV VKYGKWRGQY DVAIKMIKEG SMSEDEFIEE AKVMMNLSHE KLVQLYGVCT 481 KQRPIFIITE YMANGCLLNY LREMRHRFQT QQLLEMCKDV CEAMEYLESK QFLHRDLAAR 541 NCLVNDQGVV KVSDFGLSRY VLDDEYTSSV GSKFPVRWSP PEVLMYSKFS SKSDIWAFGV 601 LMWEIYSLGK MPYERFTNSE TAEHIAQGLR LYRPHLASEK VYTIMYSCWH EKADERPTFK 661 ILLSNILDVM DEES
• Purity: Purity 73% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BTK Bruton tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton tyrosine kinase; AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
• Gene ID: 695
• mRNA Refseq: NM_000061.3
• Protein Refseq: NP_000052.1
• MIM: 300300
• UniProt ID: Q06187