BTK

Case study 1: Wenjia Ren, 2023

Lung cancer is one of the most lethal malignant tumors in the world. Non-small cell lung cancer (NSCLC) is the most common pathological subtype. However, the molecular mechanism of NSCLC progress is still unclear. The researchers extracted the expression data of the Bruton's tyrosine kinase (BTK) gene in NSCLC tissues from the TCGA database. The results of paired t-test showed that the BTK gene was significantly underexpressed in NSCLC tissues. To further verify the above results, they detected the expression of the BTK gene in NSCLC cell lines A549, H1299, and H1650 at the RNA and protein levels by real-time fluorescent quantitative polymerase chain reaction and Western Blot analysis, respectively. The results showed that BTK was low expressed in NSCLC tissues and cells. More importantly, the expression of the BTK gene is also significantly related to the patient's age, gender, tumor range (T), lymph node invasion (N), tumor stage, and prognosis, and its expression level gradually decreases with the progress of the disease. It is speculated that BTK may be an independent prognostic factor of NSCLC. Overexpression of BTK can significantly inhibit the proliferation, migration, and invasion of NSCLC cells and can block the G0/G1 tumor cell cycle, indicating that overexpression of BTK can inhibit the growth, migration, and invasion of NSCLC cells.

Fig1. Western blot analysis of BTK expression levels in normal bronchial epithelial cell 16HBE and non-small cell lung cancer cell lines.

Fig2. Overexpression of BTK inhibits the proliferation of A549, H1299, and H1650 cells. Western blot was used to detect the overexpression efficiency of BTK in cancer cell lines.

Case study 2: Chenglong Yue, 2017

Malignant glioma is the most common primary brain tumor in adults and has a poor prognosis. However, there are no effective targeted therapies for glioma patients. Thus, the development of novel targeted therapeutics for glioma is urgently needed. In this study, the researchers examined the prognostic significance BTK expression in patients with glioma. Furthermore, they investigated the mechanism and therapeutic potential of ibrutinib in the treatment of human glioma in vitro and in vivo.

These data demonstrate that high expression of BTK is a novel prognostic marker for poor survival in patients with glioma. BTK-specific inhibitor ibrutinib effectively inhibits the proliferation, migration and invasion ability of glioma cells. Furthermore, ibrutinib can induce G1 cell-cycle arrest by regulating multiple cell cycle-associated proteins. More importantly, they found that BTK inhibition significantly blocks the degradation of IκBα and prevents the nuclear accumulation of NF-κB p65 subunit induced by EGF in glioma cells.

Fig3. HA1800 and U87 cells were treated with the indicated concentration of ibrutinib for 72 h. The cell viability was measured using CCK-8 assays.

Fig4. BTK knockdown cells were stimulated with EGF (100 ng/mL) for indicated time and then cell lysates were subjected to immunoblotting analysis using indicated antibodies.

Fig1. Canonical and alternative signaling pathways in B-cells. (Alexandra Chirino, 2023)

Fig2. BTK is engaged downstream of the constitutively activated B cell receptor (BCR) in CLL. (Benjamin L Lampson, 2018)

BTK involved in several pathways and played different roles in them. We selected most pathways BTK participated on our site, such as NF-kappa B signaling pathway, Osteoclast differentiation, Platelet activation, which may be useful for your reference. Also, other proteins which involved in the same pathway with BTK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

BTK has several biochemical functions, for example, ATP binding, identical protein binding, metal ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by BTK itself. We selected most functions BTK had, and list some proteins which have the same functions with BTK. You can find most of the proteins on our site.

BTK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with BTK here. Most of them are supplied by our site. Hope this information will be useful for your research of BTK.

GTF2I; BLNK; ebi-1521221; MAL