Active Recombinant Full Length Human CAMK2D Protein, N-His-tagged

• Cat.No.: CAMK2D-33HFL
• Product Overview: N-terminal 6Histagged, recombinant, human, full length CaM Kinase IIδ, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Calcium/calmodulin dependent protein kinase II (CaMKII) is a serine/threonine kinase which is encoded as over two dozen variants generated by alternative mRNA splicing of 4 genes. CaMKII phosphorylate target proteins in a calcium calmodulin dependent manner and play critical for gene transcription, ion channel activity, cytoskeletal function and carbohydrate metabolism. CaMKII has been implicated in memory and cell growth control as well as in the disorders of cancer, cystic fibrosis, Alzheimer's disease and epilepsy. The overexpression of a specific constitutively active CAMK2D (CaMKIId) in PC-12, P19 and NIH/3T3 cells induces neurite outgrowth.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.38mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 16527U/mg, where one unit of CaM Kinase IIδ activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 58 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MASTTTCTRF TDEYQLFEEL GKGAFSVVRR 61 CMKIPTGQEY AAKIINTKKL SARDHQKLER EARICRLLKH PNIVRLHDSI SEEGFHYLVF 121 DLVTGGELFE DIVAREYYSE ADASHCIQQI LESVNHCHLN GIVHRDLKPE NLLLASKSKG 181 AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKDP YGKPVDMWAC GVILYILLVG 241 YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT VTPEAKDLIN KMLTINPAKR ITASEALKHP 301 WICQRSTVAS MMHRQETVDC LKKFNARRKL KGAILTTMLA TRNFSAAKSL LKKPDGVKES 361 TESSNTTIED EDVKARKQEI IKVTEQLIEA INNGDFEAYT KICDPGLTAF EPEALGNLVE 421 GMDFHRFYFE NALSKSNKPI HTIILNPHVH LVGDDAACIA YIRLTQYMDG SGMPKTMQSE 481 ETRVWHRRDG KWQNVHFHRS GSPTVPIK
• Purity: Purity 90.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CAMK2D calcium/calmodulin dependent protein kinase II delta [ Homo sapiens (human) ]
• Official Symbol: CAMK2D
• Synonyms: CAMK2D; calcium/calmodulin dependent protein kinase II delta; CAMKD
• Gene ID: 817
• mRNA Refseq: NM_001221.4
• Protein Refseq: NP_001212.2
• MIM: 607708
• UniProt ID: Q13557