Active Recombinant Full Length Human CDK2 and CCNA2 co-expressed Protein, C-His,N-GST-tagged

• Cat.No.: CDK2-CCNA2-46HFL
• Product Overview: C-terminal 6His-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A. These are expressed individually by baculovirus in Sf21 insect cells. The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose. They are then complexed in vitro.

Specification

• Description: The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S transition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
• Source: Sf21
• Species: Human
• Tag: His,GST
• Protein length: Full Length
• Form: 1.076mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 184U/mg, where one unit of cdk2/cyclinA, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml histone H1 per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: cdk2 MW = 35kDa and cyclin A MW = 75kDa.
• AA Sequence: cdk2 amino acid sequence: 1 MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 61 PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS 121 HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 181 STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF 241 PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLHH 301 HHHH
  cyclinA amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MLGNSAPGPA 241 TREAGSALLA LQQTALQEDQ ENINPEKAAP VQQPRTRAAL AVLKSGNPRG LAQQQRPKTR 301 RVAPLKDLPV NDEHVTVPPW KANSKQPAFT IHVDEAEKEA QKKPAESQKI EREDALAFNS 361 AISLPGPRKP LVPLDYPMDG SFESPHTMDM SIVLEDEKPV SVNEVPDYHE DIHTYLREME 421 VKCKPKVGYM KKQPDITNSM RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR 481 GKLQLVGTAA MLLASKFEEI YPPEVAEFVY ITDDTYTKKQ VLRMEHLVLK VLTFDLAAPT 541 VNQFLTQYFL HQQPANCKVE SLAMFLGELS LIDADPYLKY LPSVIAGAAF HLALYTVTGQ 601 SWPESLIRKT GYTLESLKPC LMDLHQTYLK APQHAQQSIR EKYKNSKYHG VSLLNPPETL 661 NL
• Purity: Combined purity 43% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK2 cyclin dependent kinase 2 [ Homo sapiens (human) ]&CCNA2 cyclin A2 [ Homo sapiens (human) ]
• Official Symbol: CDK2 and CCNA2
• Synonyms: CDK2 and CCNA2; cyclin dependent kinase 2 and cyclin A2; CDKN2; p33(CDK2) and CCN1; CCNA
• Gene ID: 1017 and 890
• mRNA Refseq: NM_001798.5 and NM_001237.5
• Protein Refseq: NP_001789.2 and NP_001228.2
• MIM: 116953 and 123835
• UniProt ID: P24941 and P20248