Active Recombinant Full Length Human DAPK2 Protein, N-GST-tagged

• Cat.No.: DAPK2-62HFL
• Product Overview: N-terminal GSTtagged, recombinant, human, full length DAPK2, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-induced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependent serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNγ-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 0.937mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 105U/mg, where one unit of DAPK2 activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 70.6 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQMFQA 241 SMRSPNMEPF KQQKVEDFYD IGEELGSGQF AIVKKCREKS TGLEYAAKFI KKRQSRASRR 301 GVSREEIERE VSILRQVLHH NVITLHDVYE NRTDVVLILE LVSGGELFDF LAQKESLSEE 361 EATSFIKQIL DGVNYLHTKK IAHFDLKPEN IMLLDKNIPI PHIKLIDFGL AHEIEDGVEF 421 KNIFGTPEFV APEIVNYEPL GLEADMWSIG VITYILLSGA SPFLGDTKQE TLANITAVSY 481 DFDEEFFSQT SELAKDFIRK LLVKETRKRL TIQEALRHPW ITPVDNQQAM VRRESVVNLE 541 NFRKQYVRRR WKLSFSIVSL CNHLTRSLMK KVHLRPDEDL RNCESDTEED IARRKALHPR 601 RRSSTS
• Purity: Purity 78% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DAPK2 death associated protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DAPK2
• Synonyms: DAPK2; death associated protein kinase 2; DRP1; DRP-1
• Gene ID: 23604
• mRNA Refseq: NM_001363730.2
• Protein Refseq: NP_001350659.1
• MIM: 616567
• UniProt ID: Q9UIK4