Active Recombinant Full Length Human DCLK1 Protein, N-His-tagged

• Cat.No.: DCLK1-63HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human DCAMKL1 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.67mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 419U/mg, where one unit of DCAMKL1 activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 85 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MSFGRDMELE HFDERDKAQR YSRGSRVNGL 61 PSPTHSAHCS FYRTRTLQTL SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD 121 LTRTLSDNVN LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW 181 SVNVKTTSAS RAVSSLATAK GSPSEVRENK DFIRPKLVTI IRSGVKPRKA VRILLNKKTA 241 HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG DDDIFIACGP EKFRYQDDFL 301 LDESECRVVK STSYTKIASS SRRSTTKSPG PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS 361 PSPSPTSPGS LRKQRSSQHG GSSTSLASTK VCSSMDENDG PGEEVSEEGF QIPATITERY 421 KVGRTIGDGN FAVVKECVER STAREYALKI IKKSKCRGKE HMIQNEVSIL RRVKHPNIVL 481 LIEEMDVPTE LYLVMELVKG GDLFDAITST NKYTERDASG MLYNLASAIK YLHSLNIVHR 541 DIKPENLLVY EHQDGSKSLK LGDFGLATIV DGPLYTVCGT PTYVAPEIIA ETGYGLKVDI 601 WAAGVITYIL LCGFPPFRGS GDDQEVLFDQ ILMGQVDFPS PYWDNVSDSA KELITMMLLV 661 DVDQRFSAVQ VLEHPWVNDD GLPENEHQLS VAGKIKKHFN TGPKPNSTAA GVSVIALDHG 721 FTIKRSGSLD YYQQPGMYWI RPPLLIRRGR FSDEDATRM
• Purity: Purity 52% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DCLK1 doublecortin like kinase 1 [ Homo sapiens (human) ]
• Official Symbol: DCLK1
• Synonyms: DCLK1; doublecortin like kinase 1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• Gene ID: 9201
• mRNA Refseq: NM_001195415.2
• Protein Refseq: NP_001182344.1
• MIM: 604742
• UniProt ID: O15075