Active Recombinant Full Length Human LCK Protein, N-His-tagged

• Cat.No.: LCK-91HFL
• Product Overview: N-terminal 6Histagged, recombinant full-length human Lck expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The Lck proto-oncogene is a member of the Src-related gene family of non-receptor tyrosine kinases. It is uniquely expressed only in T-lymphocytes and NK-cells. The p56lck kinase (LCK) is a 56 KD polypeptide that is associated with a cytoplasmic face of the plasma membrane. It functions as a critical Signaling model in IL-2 signal transduction leading to T-lymphocyte activation. Specifically, it has been shown that a stable complex exists between IL-2 receptors and p56lck and that treatment of T-lymphocytes with IL-2 promotes p56lck kinase activity and rapid phosphorylation of cellular substrates. In addition, p56lck has been found to function as a signal transducer for antigen activated CD4 and CG8 T-cell receptors and plays a role in thymic maturation of certain T-cells. The CD45 antigen, a receptor/protein tyrosine phosphatase of T-cells, also interacts with p56lck.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.99mg/ml of enzyme in 50mM Tris/HCl, pH7.5, 0.1mM EGTA, 150mM NaCl, 1mM benzamidine, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5% glycerol. Frozen solution.
• Bio-activity: 394U/mg, where one unit of Lck, active activity is defined as 1nmol phosphate incorporated into 250μM cdc 2 peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59 kDa
• AA Sequence: 1 MHHHHHHMGC GCSSHPEDDW MENIDVCENC HYPIVPLDGK GTLLIRNGSE VRDPLVTYEG 61 SNPPASPLQD NLVIALHSYE PSHDGDLGFE KGEQLRILEQ SGEWWKAQSL TTGQEGFIPF 121 NFVAKANSLE PEPWFFKNLS RKDAERQLLA PGNTHGSFLI RESESTAGSF SLSVRDFDQN 181 QGEVVKHYKI RNLDNGGFYI SPRITFPGLH ELVRHYTNAS DGLCTRLSRP CQTQKPQKPW 241 WEDEWEVPRE TLKLVERLGA GQFGEVWMGY YNGHTKVAVK SLKQGSMSPD AFLAEANLMK 301 QLQHQRLVRL YAVVTQEPIY IITEYMENGS LVDFLKTPSG IKLTINKLLD MAAQIAEGMA 361 FIEERNYIHR DLRAANILVS DTLSCKIADF GLARLIEDNE YTAREGAKFP IKWTAPEAIN 421 YGTFTIKSDV WSFGILLTEI VTHGRIPYPG MTNPEVIQNL ERGYRMVRPD NCPEELYQLM 481 RLCWKERPED RPTFDYLRSV LEDFFTATEG QYQPQP
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LCK LCK proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: LCK
• Synonyms: LCK; LCK proto-oncogene, Src family tyrosine kinase; LSK; YT16; IMD22; p56lck; pp58lck
• Gene ID: 3932
• mRNA Refseq: NM_005356.5
• Protein Refseq: NP_005347.3
• MIM: 153390
• UniProt ID: P06239