Active Recombinant Full Length Human MARK2 Protein, N-His-tagged

• Cat.No.: MARK2-125HFL
• Product Overview: N-terminal 6Histagged, recombinant, full-length, human PAR-1B alpha, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA.

Specification

• Description: PAR-1Bα Protein has a role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.5215mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1670U/mg, where one unit of PAR-1B-alpha, active activity is defined as 1nmol phosphate incorporated into 100μM CHKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 81.6 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGMIRGRNSA TSADEQPHIG NYRLLKTIGK 61 GNFAKVKLAR HILTGKEVAV KIIDKTQLNS SSLQKLFREV RIMKVLNHPN IVKLFEVIET 121 EKTLYLVMEY ASGGEVFDYL VAHGRMKEKE ARAKFRQIVS AVQYCHQKFI VHRDLKAENL 181 LLDADMNIKI ADFGFSNEFT FGNKLDTFCG SPPYAAPELF QGKKYDGPEV DVWSLGVILY 241 TLVSGSLPFD GQNLKELRER VLRGKYRIPF YMSTDCENLL KKFLILNPSK RGTLEQIMKD 301 RWMNVGHEDD ELKPYVEPLP DYKDPRRTEL MVSMGYTREE IQDSLVGQRY NEVMATYLLL 361 GYKSSELEGD TITLKPRPSA DLTNSSAPSP SHKVQRSVSA NPKQRRFSDQ AGPAIPTSNS 421 YSKKTQSNNA ENKRPEEDRE SGRKASSTAK VPASPLPGLE RKKTTPTPST NSVLSTSTNR 481 SRNSPLLERA SLGQASIQNG KDSTAPQRVP VASPSAHNIS SSGGAPDRTN FPRGVSSRST 541 FHAGQLRQVR DQQNLPYGVT PASPSGHSQG RRGASGSIFS KFTSKFVRRN LNEPESKDRV 601 ETLRPHVVGS GGNDKEKEEF REAKPRSLRF TWSMKTTSSM EPNEMMREIR KVLDANSCQS 661 ELHEKYMLLC MHGTPGHEDF VQWEMEVCKL PRLSLNGVRF KRISGTSMAF KNIASKIANE 721 LKL
• Purity: Purity 31.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MARK2 microtubule affinity regulating kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MARK2
• Synonyms: MARK2; microtubule affinity regulating kinase 2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
• Gene ID: 2011
• mRNA Refseq: NM_001039469.3
• Protein Refseq: NP_001034558.2
• MIM: 600526
• UniProt ID: Q7KZI7