Active Recombinant Full Length Human PAK3 Protein, C-His-tagged

• Cat.No.: PAK3-124HFL
• Product Overview: C-terminal 6Histagged, recombinant, human full length, PAK3 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+-NTA agarose.

Specification

• Description: PAK3 (P21 (RAC1) Activated Kinase 3) is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. PAK3 plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. PAK3 also plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity, and acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.817mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 20mM β-glycerophosphate, 10mM NaF, 0.25mM sodium orthovanadate, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 7976U/mg, where one unit of PAK3 activity is defined as 1nmol phosphate incorporated into PAKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.2 kDa
• AA Sequence: 1 MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN 61 KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTPDLYGSQM CPGKLPEGIP EQWARLLQTS 121 NITKLEQKKN PQAVLDVLKF YDSKETVNNQ KYMSFTSGDK SAHGYIAAHP SSTKTASEPP 181 LAPPVSEEED EEEEEEEDEN EPPPVIAPRP EHTKSIYTRS VVESIASPAV PNKEVTPPSA 241 ENANSSTLYR NTDRQRKKSK MTDEEILEKL RSIVSVGDPK KKYTRFEKIG QGASGTVYTA 301 LDIATGQEVA IKQMNLQQQP KKELIINEIL VMRENKNPNI VNYLDSYLVG DELWVVMEYL 361 AGGSLTDVVT ETCMDEGQIA AVCRECLQAL DFLHSNQVIH RDIKSDNILL GMDGSVKLTD 421 FGFCAQITPE QSKRSTMVGT PYWMAPEVVT RKAYGPKVDI WSLGIMAIEM VEGEPPYLNE 481 NPLRALYLIA TNGTPELQNP ERLSAVFRDF LNRCLEMDVD RRGSAKELLQ HPFLKLAKPL 541 SSLTPLIIAA KEAIKNSSRH HHHHH
• Purity: Purity 75% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PAK3 p21 (RAC1) activated kinase 3 [ Homo sapiens (human) ]
• Official Symbol: PAK3
• Synonyms: PAK3; p21 (RAC1) activated kinase 3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; XLID30; PAK3beta; beta-PAK
• Gene ID: 5063
• mRNA Refseq: NM_001128166.3
• Protein Refseq: NP_001121638.1
• MIM: 300142
• UniProt ID: O75914