Active Recombinant Full Length Human PIM1 Protein, N-GST-tagged

• Cat.No.: PIM1-145HFL
• Product Overview: N-terminal GSTtagged recombinant human full length Pim-1, expressed in E. coli cells. Purified using gluthathione agarose.

Specification

• Description: Protein serine/threonine kinase PIM1 (pim-1-oncogene) is a member of a small family of highly homologous kinases, including PIM1, PIM2, and PIM3, and all members are expressed in the thymus. Overexpression of each of these members can promote lymphomagenesis in mice. PIM1 associates with Myb and phosphorylates the Myb DNA binding domain, suggesting that it regulates Myb protein activity by direct phosphorylation. Recently four proto-oncogenes PIM1, cMYC, PAX-5 and RhoH/TTF were found hypermutated in diffuse large B cell lymphoma (DLBCL) with >50% of cases carrying at least two mutated genes.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 1.872mg/ml of enzyme in 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3495U/mg, where one unit of Pim-1 activity is defined as 1nmol phosphate incorporated into 100μM (KKRNRTLTV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 62 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM LLSKINSLAH 241 LRAAPCNDLH ATKLAPGKEK EPLESQYQVG PLLGSGGFGS VYSGIRVSDN LPVAIKHVEK 301 DRISDWGELP NGTRVPMEVV LLKKVSSGFS GVIRLLDWFE RPDSFVLILE RPEPVQDLFD 361 FITERGALQE ELARSFFWQV LEAVRHCHNC GVLHRDIKDE NILIDLNRGE LKLIDFGSGA 421 LLKDTVYTDF DGTRVYSPPE WIRYHRYHGR SAAVWSLGIL LYDMVCGDIP FEHDEEIIRG 481 QVFFRQRVSS ECQHLIRWCL ALRPSDRPTF EEIQNHPWMQ DVLLPQETAE IHLHSLSPGP 541 SK
• Purity: Purity 85.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIM1 Pim-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PIM1
• Synonyms: PIM1; Pim-1 proto-oncogene, serine/threonine kinase; PIM
• Gene ID: 5292
• mRNA Refseq: NM_002648.4
• Protein Refseq: NP_002639.1
• MIM: 164960
• UniProt ID: P11309