PIM1

  • Official Full Name

    pim-1 oncogene

  • Overview

    PIM-1 is a member of the serine/threonine kinase PIM oncogene family. PIM-1 has been implicated in lymphomagenesis, cell proliferation, apoptosis, differentiation and tumourigenesis. The PIM-1 protein kinase is upregulated in prostate cancer.
  • Synonyms

    PIM1; pim-1 oncogene; PIM; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Assay Kits
  • Human
  • Human
  • Mouse
  • Rat
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Insect (sf21)
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • His|GST
  • Myc
  • DDK
  • N/A
  • N
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human PIM1-145HFL Active Recombinant Full Length Human PIM1 Protein, N-GST-tagged E.coli GST Full Length
Human PIM1-310H Recombinant Human PIM1 Mammalian Cell His
Human PIM1-7906H Recombinant Human PIM1 protein, His & GST-tagged E.coli His/GST Tyr129~Leu268 (Accession # P11309)
Human PIM1-7553H Recombinant Human PIM1, His-tagged E.coli His 38-290aa
Human PIM1-726H Recombinant Human Pim-1 Oncogene, GST-His Sf9 Insect Cell GST
Human PIM1-392H Recombinant Human Pim-1 Oncogene, GST-tagged, Active Sf9 Insect Cell GST
Human PIM1-1288H Recombinant Human Pim-1 Oncogene, His-tagged Insect Cell His
Human PIM1-3181HCL Recombinant Human PIM1 293 Cell Lysate HEK293 N/A
Human PIM1-2714H Recombinant Human PIM1 protein, His-tagged E.coli His 244-313 aa
Human PIM1-959HFL Recombinant Full Length Human PIM1 Protein, C-Flag-tagged Mammalian cells Flag
Human PIM1-301405H Recombinant Human PIM1 protein, GST-tagged E.coli GST His244-Lys313
Human PIM1-4898H Recombinant Human PIM1 Protein (Ser98-Lys410), His tagged E.coli His Ser98-Lys410
Human PIM1-2938HF Active Recombinant Full Length Human PIM1 Protein, His-tagged Insect (sf21) His 1-313
Human PIM1-1680H Recombinant Human PIM1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human PIM1-0920H Recombinant Human PIM1 Protein (A14-K313), His tagged E.coli His A14-K313
Human PIM1-4899H Recombinant Human PIM1 Protein (Ser98-Lys313), N-His tagged E.coli N-His Ser98-Lys313
Human PIM1-0919H Recombinant Human PIM1 Protein (A14-K313), Tag Free E.coli No tag A14-K313
Human PIM1-1680H-B Recombinant Human PIM1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Pim1-7907M Recombinant Mouse Pim1 protein, His-tagged E.coli His Tyr122~Leu261
Mouse Pim1-4868M Recombinant Mouse Pim1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Rat PIM1-4465R Recombinant Rat PIM1 Protein Mammalian Cell His
Rat Pim1-7908R Recombinant Rat Pim1 protein, His-tagged E.coli His Tyr38~Leu177 (Accession # P26794)
Rat PIM1-4125R-B Recombinant Rat PIM1 Protein Pre-coupled Magnetic Beads HEK293
Rat PIM1-4125R Recombinant Rat PIM1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish PIM1-9086Z Recombinant Zebrafish PIM1 Mammalian Cell His
Kit-1619 PIM1 Kinase Binding Assay Kit N/A
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • PIM1 Related Signal Pathway

What is PIM1 protein?

PIM1 (Pim-1 proto-oncogene, serine/threonine kinase) gene is a protein coding gene which situated on the short arm of chromosome 6 at locus 6p21. PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. The PIM1 protein is consisted of 313 amino acids and its molecular mass is approximately 35.7 kDa.

What is the function of PIM1 protein?

PIM1 is a proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. It regulates a variety of cellular processes such as cell proliferation, survival, and differentiation. Pim-1 phosphorylates and regulates multiple substrate proteins, including transcription factors, cyclins, and other kinases and signaling molecules. In addition, Pim-1 is also involved in physiological and pathological processes such as tumor development, immune regulation, and cardiovascular disease.

PIM1-9.jpg

Fig1. Working model showing potential roles of PIM1 in hepatic lineage reprogramming. (Yangyang Yuan, 2022)

PIM1 Related Signaling Pathway

PIM1 performs its function by phosphorylating a variety of substrates, such as transcription factors, cell cycle regulatory proteins, and apoptosis-related proteins. The role of Pim-1 in cell signal transduction is mainly reflected in the following aspects: promoting cell cycle process, inhibiting cell apoptosis, regulating metabolism and influencing cell differentiation. In addition, Pim-1 also interacts with signaling pathways related to tumorigenesis and development, including PI3K/AKT, MAPK, mTOR, etc., thus playing an important role in cancer.

PIM1 Related Diseases

PIM1 is associated with a variety of diseases, especially in the field of cancer. Overexpression or enhanced activity of Pim-1 has been observed in many types of cancer, including prostate, breast, lung, and lymphoma. In addition, PIM1's role in cell survival and metabolic regulation also suggests that it may be associated with other diseases, such as cardiovascular disease and certain metabolic diseases.

Bioapplications of PIM1

Although clinical drugs that directly target PIM1 are not yet fully approved, several Pim inhibitors have entered clinical trials to evaluate their potential in the treatment of blood cancers and solid tumors. These inhibitors are typically multikinase inhibitors that simultaneously target members of the Pim family as well as other related signaling pathways.

High Purity

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PIM1-2.jpg

Fig2. Activity Data. (PIM1-2938HF)

Case study 1: Ashley Y Gao, 2022

Cellular senescence is emerging as a driver of idiopathic pulmonary fibrosis (IPF), a progressive and fatal disease with limited effective therapies. The senescence-associated secretory phenotype (SASP), involving the release of inflammatory cytokines and profibrotic growth factors by senescent cells, is thought to be a product of multiple cell types in IPF, including lung fibroblasts. The purpose of this study was to assess the role of Pim-1 kinase as a driver of NF-κB-induced production of inflammatory cytokines from low-passage IPF fibroblast cultures displaying markers of senescence.

The results demonstrate that Pim-1 kinase phosphorylates p65/RelA, activating NF-κB activity and enhancing IL-6 production, which in turn amplifies the expression of PIM1, generating a positive feedback loop. In addition, targeting Pim-1 kinase with a small molecule inhibitor dramatically inhibited the expression of a broad array of cytokines and chemokines in IPF-derived fibroblasts. These findings highlight the therapeutic potential of targeting Pim-1 kinase to reprogram the secretome of senescent fibroblasts and halt IPF progression.

PIM1-3.jpg

Fig1. Proviral integration site for Moloney murine leukemia virus 1 (Pim-1) regulates phosphorylation and activity of p65/RelA.

PIM1-4.jpg
Fig2. Proviral Integration site for Moloney murine leukemia virus 1 (Pim-1) promotes lung fibroblast premature senescence.

Case study 2: Yangyang Yuan, 2022

Protein kinase-mediated phosphorylation plays a critical role in many biological processes. However, the identification of key regulatory kinases is still a great challenge. Here, the researchers develop a trans-omics-based method, central kinase inference, to predict potentially key kinases by integrating quantitative transcriptomic and phosphoproteomic data. Using known kinases associated with anti-cancer drug resistance, the accuracy of our method denoted by the area under the curve is 5.2% to 29.5% higher than Kinase-Substrate Enrichment Analysis.

Further experiments reveal that a serine/threonine kinase, PIM1, promotes hepatic conversion and protects human dermal fibroblasts from reprogramming-induced ferroptosis and cell cycle arrest. This study not only reveals new regulatory kinases, but also provides a helpful method that might be extended to predict central kinases involved in other biological processes.

PIM1-5.jpg

Fig3. Experimental analysis of PIM1 roles in hepatic reprogramming. Immunoblotting of PIM1 in HDFs infected with FHH for the indicated number of days.

PIM1-6.jpg
Fig4. PAS staining and DiI-ac-LDL uptake assay of HDFs infected with FHH and either PIM1 shRNA or non-targeted shRNA for 12 days.
PIM1-7.jpg

Fig1. Pim-1 phosphorylates p65/RelA, supporting NF-κB transcriptional activity that stimulates IL-6 synthesis. (Ashley Y Gao, 2022)

PIM1-8.jpg

Fig2. The mechanism by which PIM1 mediates ZIKV replication. (Fanghang Zhou, 2021)

PIM1 involved in several pathways and played different roles in them. We selected most pathways PIM1 participated on our site, such as Jak-STAT signaling pathway, MicroRNAs in cancer, Acute myeloid leukemia, which may be useful for your reference. Also, other proteins which involved in the same pathway with PIM1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Jak-STAT signaling pathwayBCL2L1;FHL1;CCND3;IL2RA;MCL1A;PRLR;STAT5B;IFNA8;IL23R
MicroRNAs in cancerZEB1;NRAS;CYP24A1;MET;RASSF1;PRKCB;CCND1;PDGFRA;TIMP3
Acute myeloid leukemiaPIK3CA;RUNX1;RARA;FLT3;AKT3;BRAF;IKBKB;SFPI1;TCF7L2

PIM1 has several biochemical functions, for example, ATP binding, manganese ion binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by PIM1 itself. We selected most functions PIM1 had, and list some proteins which have the same functions with PIM1. You can find most of the proteins on our site.

Function Related Protein
ATP bindingMYHZ1.2;PRKACAB;ACTA1A;AGAP2;DHX33;KIF2B;KIF26A;FASTK;MAP2K3
manganese ion bindingNPEPL1;PAPOLA;NUDT16;GYLTL1B;ME1;GALNT2;PPM1M;DCP2;DYRK2
protein bindingCRNKL1;LGR6;SERPINA1;TUBG1;MTA2;FEM1B;PSMA1;FASTKD3;ALAS2
protein serine/threonine kinase activityRAF1;CSNK1D;CSNK1DA;RIPK1;STK11;ALPK1;AAK1;PDIK1L;CLK3
ribosomal small subunit bindingNME1;DDX3X;DHX29;PIM1;PTCD3;ERAL1;CPEB2;MTIF3;NPM1
transcription factor bindingREST;PPRC1;NR0B2;STK4;RBFOX2;HES3;PIAS2;TBP;CTNNB1

PIM1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PIM1 here. Most of them are supplied by our site. Hope this information will be useful for your research of PIM1.

RPS19; TFPT; FH; FXR2; NHLH1; BEND7

Research Area

Related articles

Perera, GK; Ainali, C; et al. Integrative Biology Approach Identifies Cytokine Targeting Strategies for Psoriasis. SCIENCE TRANSLATIONAL MEDICINE 6:-(2014).
Dourado, IBZ; Batista, WL; et al. Dual localization of Mdj1 in pathogenic fungi varies with growth temperature. MEDICAL MYCOLOGY 52:187-195(2014).
  • Q&As
  • Reviews

Q&As (7)

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What are the main substrates of PIM1 and how does their interaction affect cellular processes? 01/28/2022

PIM1 phosphorylates a variety of substrates, including BAD, c-Myc, and eIF4B. Phosphorylation of BAD inhibits its pro-apoptotic function, while phosphorylation of c-Myc and eIF4B enhances their transcriptional and translational activities, promoting cell survival and protein synthesis, respectively.

How is PIM1 activity regulated by signaling pathways? 05/01/2021

PIM1 activity is regulated by several signaling pathways, including the PI3K/AKT and JAK/STAT pathways. Activation of these pathways leads to the phosphorylation and activation of PIM1, while inhibition of these pathways can result in reduced PIM1 activity.

What is the potential of PIM1 as a prognostic marker or therapeutic target in different cancer types? 11/24/2020

Further research is needed to assess the potential of PIM1 as a prognostic marker and therapeutic target in different cancer types. Understanding the specific roles of PIM1 in various cancers and its interactions with other signaling pathways will help determine its clinical relevance and potential utility in personalized cancer treatment strategies.

What role does PIM1 play in cancer development? 02/13/2020

PIM1 has been implicated in promoting cell proliferation, survival, and resistance to apoptosis, contributing to cancer development. Its overexpression has been observed in various cancer types and is associated with poor prognosis. PIM1 also interacts with oncogenic signaling pathways, further promoting tumor growth and progression.

Are there any potential therapeutic strategies targeting PIM1? 01/23/2018

Several small molecule inhibitors targeting PIM1 kinase activity have been developed and show promise as potential therapeutics. These inhibitors can selectively inhibit PIM1 activity and have demonstrated anti-tumor effects in preclinical studies. Combination therapy with PIM1 inhibitors and other targeted drugs may also be a viable approach for treating PIM1-driven cancers.

How does PIM1 regulate cell cycle progression and proliferation? 03/17/2017

PIM1 regulates cell cycle progression and proliferation by phosphorylating key substrates, such as p27 and CDC25A, which control the activity of cyclin-dependent kinases (CDKs). This phosphorylation promotes the degradation of p27 and stabilizes CDC25A, leading to enhanced CDK activity and cell cycle progression.

How is PIM1 expression regulated at the transcriptional level? 02/16/2016

PIM1 expression can be regulated by various factors, including cytokines, growth factors, and oncogenic signals. These factors can activate specific transcription factors, such as STAT3 and NF-κB, which bind to the PIM1 promoter and enhance its transcriptional activity, resulting in increased PIM1 expression.

Customer Reviews (3)

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Reviews
01/30/2022

    With the combination of this protein reagent's instructions, I can easily accomplish experimental design and operation.

    04/29/2020

      With consistent and reliable results, this protein reagent is ideal for research projects that require replicability.

      01/02/2020

        Following the use of this protein reagent, the reproducibility of my experiments has significantly improved, and I am highly satisfied.

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