| Description : |
Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.13mg/ml of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1354U/mg, where one unit of PIP4K2α activity is defined as 1nmol phosphatidylinositol 4,5- bisphosphate (PIP2) formed per minute at room temperature with a final ATP concentration of 200μM. |
| Molecular Mass : |
51 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRRAQLV MATPGNLGSS VLASKTKTKK 61 KHFVAQKVKL FRASDPLLSV LMWGVNHSIN ELSHVQIPVM LMPDDFKAYS KIKVDNHLFN 121 KENMPSHFKF KEYCPMVFRN LRERFGIDDQ DFQNSLTRSA PLPNDSQARS GARFHTSYDK 181 RYIIKTITSE DVAEMHNILK KYHQYIVECH GITLLPQFLG MYRLNVDGVE IYVIVTRNVF 241 SHRLSVYRKY DLKGSTVARE ASDKEKAKEL PTLKDNDFIN EGQKIYIDDN NKKVFLEKLK 301 KDVEFLAQLK LMDYSLLVGI HDVERAEQEE VECEENDGEE EGESDGTHPV GTPPDSPGNT 361 LNSSPPLAPG EFDPNIDVYG IKCHENSPRK EVYFMAIIDI LTHYDAKKKA AHAAKTVKHG 421 AGAEISTVNP EQYSKRFLDF IGHILT |
| Purity : |
Purity 96% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
