Active Recombinant Full Length Human PRKACB Protein, N-His-tagged

• Cat.No.: PRKACB-150HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full length, human PKACβ, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: PKAcb is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates Signaling though cAMP. cAMP signaling is important to a number of processes, including cell proliferation and differentiation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.33 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2075 U/mg, where one unit of PKACβ activity is defined as 1 nmol phosphate incorporated into 30 μM Long S6 peptide per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 45 kDa
• Purity: 75% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKACB protein kinase cAMP-activated catalytic subunit beta [ Homo sapiens (human) ]
• Official Symbol: PRKACB
• Synonyms: PRKACB; protein kinase cAMP-activated catalytic subunit beta; CAFD2; PKACB; PKA C-beta
• Gene ID: 5567
• mRNA Refseq: NM_001242857.3
• Protein Refseq: NP_001229786.1
• MIM: 176892
• UniProt ID: P22694