Recombinant Human PRKACB 293 Cell Lysate

Cat.No. : PRKACB-2868HCL
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Description : Antigen standard for protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : PRKACB protein kinase, cAMP-dependent, catalytic, beta [ Homo sapiens ]
Official Symbol : PRKACB
Synonyms : PRKACB; protein kinase, cAMP-dependent, catalytic, beta; cAMP-dependent protein kinase catalytic subunit beta; PKACb; PKA C-beta; protein kinase A catalytic subunit beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; PKACB; MGC9320; MGC41879; DKFZp781I2452;
Gene ID : 5567
mRNA Refseq : NM_001242857
Protein Refseq : NP_001229786
MIM : 176892
UniProt ID : P22694
Chromosome Location : 1p36.1
Pathway : AMPK signaling, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem;
Function : ATP binding; cAMP-dependent protein kinase activity; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding;

Not For Human Consumption!

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