Active Recombinant Full Length Human SRC Protein, N-His-tagged
Cat.No. : | SRC-171HFL |
Product Overview : | N-Terminal 6Histagged, recombinant, full-length, human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. |
- Specification
- Gene Information
- Related Products
Description : | Src family tyrosine kinases are non-receptor tyrosine kinases that are functionally linked to some receptor-type tyrosine kinases, G-protein-coupled receptors, cytokine receptors, and cell adhesion molecules/receptors. Src family tyrosine kinases are membrane-associated proteins having Src homology 2 (SH2) and SH3 domains, which are important for protein-protein interaction. The activity of Src family tyrosine kinases is regulated through intramolecular interaction between the SH2 domain and the phosphorylated tyrosine at the C-terminal regulatory site (Tyr-527). |
Source : | Sf21 |
Species : | Human |
Tag : | His |
Protein length : | Full Length |
Form : | 1.128mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 5% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : | 1022U/mg, where one unit of Src activity is defined as 1nmol phosphate incorporated into 250μM cdc2 substrate peptide (cat# 12-140) per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : | 61.7 kDa |
AA Sequence : | 1 MSYYHHHHHH DEFMGSNKSK PKDASQRRRS LEPAENVHGA GGGAFPASQT PSKPASADGH 61 RGPSAAFAPA AAEPKLFGGF NSSDTVTSPQ RAGPLAGGVT TFVALYDYES RTETDLSFKK 121 GERLQIVNNT EGDWWLAHSL STGQTGYIPS NYVAPSDSIQ AEEWYFGKIT RRESERLLLN 181 AENPRGTFLV RESETTKGAY CLSVSDFDNA KGLNVKHYKI RKLDSGGFYI TSRTQFNSLQ 241 QLVAYYSKHA DGLCHRLTTV CPTSKPQTQG LAKDAWEIPR ESLRLEVKLG QGCFGEVWMG 301 TWNGTTRVAI KTLKPGTMSP EAFLQEAQVM KKLRHEKLVQ LYAVVSEEPI YIVTEYMSKG 361 SLLDFLKGET GKYLRLPQLV DMAAQIASGM AYVERMNYVH RDLRAANILV GENLVCKVAD 421 FGLARLIEDN EYTARQGAKF PIKWTAPEAA LYGRFTIKSD VWSFGILLTE LTTKGRVPYP 481 GMVNREVLDQ VERGYRMPCP PECPESLHDL MCQCWRKEPE ERPTFEYLQA FLEDYFTSTE 541 PQYQPGENL |
Purity : | Purity 95.3% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 1 year at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ] |
Official Symbol : | SRC |
Synonyms : | SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src |
Gene ID : | 6714 |
mRNA Refseq : | NM_005417.5 |
Protein Refseq : | NP_005408.1 |
MIM : | 190090 |
UniProt ID : | P12931 |
Products Types
◆ Recombinant Protein | ||
SRC-0402H | Recombinant Human SRC Protein (G2-L536), Tag Free | +Inquiry |
SRC-2094H | Recombinant Human SRC Protein, His (Fc)-Avi-tagged | +Inquiry |
SRC-004H | Active Recombinant Human SRC Protein, GST-tagged | +Inquiry |
Src-1367M | Recombinant Mus musculus Src Protein (V85-L535), His-tagged | +Inquiry |
Src-6122M | Recombinant Mouse Src Protein, Myc/DDK-tagged | +Inquiry |
◆ Native Protein | ||
SRC-29697TH | Native Human SRC | +Inquiry |
◆ Lysates | ||
SRC-487HCL | Recombinant Human SRC cell lysate | +Inquiry |
SRC-585MCL | Recombinant Mouse SRC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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