SRC

  • Official Full Name

    v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

  • Overview

    This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
  • Synonyms

    SRC; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; ASV; SRC1; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; EC 2.7.10.2;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Native Proteins
  • Protein Pre-coupled Magnetic Beads
  • Assay Kits
  • Chicken
  • Human
  • Human
  • Mouse
  • Mus musculus
  • Rhesus Macaque
  • RSV
  • Zebrafish
  • Baculovirus-Insect cells
  • E.coli
  • HEK293
  • HEK293T
  • Insect (sf21)
  • Insect cell
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Sf21
  • Sf9 Insect Cell
  • DDK
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • His|GST
  • Myc
  • Myc|DDK
  • N/A
  • N
  • FLAG
  • No tag
  • Tag Free
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human SRC-533H Active Recombinant Human SRC protein, His-tagged Insect Cell His
Human SRC-1100H Active Recombinant Human SRC protein, GST-tagged E.coli GST
Human SRC-575H Active Recombinant Human SRC, GST-tagged E.coli GST
Human SRC-1358H Active Recombinant Human SRC protein, His/GST-tagged Insect Cell His/GST
Human SRC-231H Active Recombinant Human SRC(1-530) Protein, N-His-tagged Sf21 His 1-530 aa
Human SRC-170HFL Active Recombinant Full Length Human SRC(T341M) Mutation Protein, N-His-tagged Sf21 His Full Length
Human SRC-171HFL Active Recombinant Full Length Human SRC Protein, N-His-tagged Sf21 His Full Length
Human SRC-381H Recombinant Human SRC Mammalian Cell His
Human SRC-2947H Recombinant Human SRC, GST-tagged E.coli GST C-term-350aa
Human SRC-191H Recombinant Human SRC, GST-tagged E.coli GST
Human SRC-433H Recombinant Human SRC, GST-tagged, Active Sf9 Insect Cell GST
Human SRC-22H Recombinant Human SRC protein, His-tagged E.coli His
Human SRC-192H Recombinant Human SRC, SH3 Domain, GST-tagged E.coli GST
Human SRC-79H Recombinant Human SRC protein, Flag-tagged, Biotinylated Insect Cell Flag
Human SRC-487HCL Recombinant Human SRC cell lysate Insect Cell N/A
Human SRC-2747H Recombinant Human SRC Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human SRC-1923H Recombinant Human SRC Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human SRC-407HFL Recombinant Full Length Human SRC Protein, C-Flag-tagged Mammalian cells Flag
Human SRC-004H Active Recombinant Human SRC Protein, GST-tagged Insect cell GST 1-536
Human SRC-1750HF Active Recombinant Full Length Human SRC Protein, GST-tagged Insect (sf21) GST 1-536(end)
Human SRC-3525H Recombinant Human SRC protein, His-tagged E.coli His 2-536aa
Human SRC2592H Recombinant Human Src (255-524) Protein
Human SRC-6766HF Active Recombinant Full Length Human SRC Protein, DDK-tagged, Biotinylated Insect (sf21) DDK 1-536(end)
Human SRC-29697TH Native Human SRC E.coli N/A
Human SRC-3526H Recombinant Human SRC protein E.coli N/A 2-536aa
Human SRC-10H Active Recombinant Human SRC Protein (Full Length), N-FLAG Insect Cell N-FLAG Full Length
Human SRC-6350H Recombinant Human SRC Protein (Met1-Leu536), N-His tagged E.coli N-His Met1-Leu536
Human SRC-2094H-B Recombinant Human SRC Protein Pre-coupled Magnetic Beads HEK293
Human SRC-2094H Recombinant Human SRC Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human SRC-0402H Recombinant Human SRC Protein (G2-L536), Tag Free Insect Cell No tag G2-L536
Human SRC-0403H Recombinant Human SRC Protein (G2-L536), His/Flag tagged Insect Cell His/Flag G2-L536
Human SRC-0186H Recombinant Human SRC Protein (Full Length), N-His-tagged Baculovirus-Insect cells N-His Full Length
Human SRC-6351H Recombinant Human SRC Protein (Tyr187-Leu536), N-His tagged E.coli N-His Tyr187-Leu536
Mouse Src-593M Recombinant Mouse Rous Sarcoma Oncogene, Domain E.coli N/A
Mouse Src-99M Recombinant Mouse Rous Sarcoma Oncogene, SH3 Domain E.coli N/A
Mouse Src-907M Active Recombinant Mouse Src protein(Met1-Leu535), His&GST-tagged Insect Cells N-His&GST Met1-Leu535
Mouse SRC-585MCL Recombinant Mouse SRC cell lysate Insect Cell N/A
Mouse Src-6122M Recombinant Mouse Src Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mus musculus Src-1367M Recombinant Mus musculus Src Protein (V85-L535), His-tagged Insect Cell His V85-L535
Mus musculus Src-1368M Recombinant Mus musculus Src Protein (V85-L535) Insect Cell Tag Free V85-L535
Rhesus Macaque SRC-4460R Recombinant Rhesus monkey SRC Protein, His-tagged Mammalian Cell His
Rhesus Macaque SRC-4276R-B Recombinant Rhesus Macaque SRC Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque SRC-4276R Recombinant Rhesus Macaque SRC Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
RSV Src-546R Active Recombinant RSV Src, GST-tagged Sf9 Insect Cell GST
Zebrafish SRC-1128Z Recombinant Zebrafish SRC Mammalian Cell His
Chicken SRC-7007C Recombinant Chicken SRC Mammalian Cell His
Kit-0800 c-Src Kinase (Human) Assay/Inhibitor Screening Assay Kit N/A
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • SRC Related Signal Pathway

What is SRC protein?

SRC (SRC proto-oncogene, non-receptor tyrosine kinase) gene is a protein coding gene which situated on the long arm of chromosome 20 at locus 20q11. This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. The SRC protein is consisted of 536 amino acids and its molecular mass is approximately 59.8 kDa.

What is the function of SRC protein?

SRC is a non-receptor tyrosine kinase belonging to the SRC family of kinases (SFKs). SRC protein plays a variety of biological roles in the cell. SRC promotes cell proliferation and prolongs cell survival by phosphorylating and activating a variety of downstream effector molecules, such as transcription factors and cell cycle regulatory proteins. SRC kinase plays a key role in regulating cytoskeletal remodeling and the formation of cell adhesion sites, which affects the cell's ability to migrate and adhere to the external environment. SRC is involved in the process of cell differentiation by influencing the activity of specific transcription factors and the interaction of extracellular matrix, especially in the differentiation of bone marrow cells and epithelial cells.

Modes of Src mechanoactivation and mechanosignalling.jpg

Fig1. Modes of Src mechanoactivation and mechanosignalling. (Lenka Koudelková, 2021)

SRC Related Signaling Pathway

SRC kinases are involved in several signaling pathways, including integrin signaling pathways, growth factor signaling pathways, and hormone receptor signaling pathways. By activating a variety of substrates downstream, SRC influences cellular behavior and physiological responses. SRC protein can activate RAS-RAF-MAPK signaling pathway and promote cell proliferation and survival. Specifically, SRC proteins can bind and activate RAS or RAF kinases, which further activate the MAPK signaling pathway and promote cell proliferation and survival. SRC protein can also activate the PI3K-AKT signaling pathway, promoting cell proliferation and survival. SRC protein can also activate the PLC-γ signaling pathway and promote cell proliferation and differentiation. The SRC protein can also activate the FAK signalling pathway, promoting cell migration and invasion.

SRC Related Diseases

Src proteins have been extensively studied for their role in a variety of cancers, including breast, colon, pancreatic, lung, ovarian, and central nervous system cancers. Src kinase plays a role in cardiac development and function, and its abnormal expression or activity is associated with the development of cardiovascular diseases, including myocardial hypertrophy, atherosclerosis, and hypertension. Src kinase is involved in regulating the activity of immune cells, including macrophage-mediated inflammatory responses, and is associated with inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease. Src proteins play a role in the cell's immune response to pathogen infections, including antiviral and antibacterial immune responses. Other diseases include neurodegenerative diseases, diabetes, osteoporosis and other diseases related.

Bioapplications of SRC

Src proteins play a key role in the development, growth, proliferation, metastasis and angiogenesis of a variety of cancers. Therefore, Src protein is an important target for cancer therapy. Research and development of inhibitors that target Src, such as small molecule drugs and antibody drugs, have potential applications for the treatment of cancer. Src kinase is a drug target, and the study of its structure and mechanism is helpful to develop new therapeutic strategies. By understanding the regulatory mechanism of Src kinase activity, more effective drugs can be designed to interfere with its function and thus treat related diseases.

High Purity

SDS-PAGE (SRC-533H).jpg

Fig1. SDS-PAGE (SRC-533H)

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SDS-PAGE (SRC-1100H).jpg

Fig2. SDS-PAGE (SRC-1100H)

Case Study 1: Xianteng Wang, 2023

Chemotherapy resistance and treatment failure hinder clinical cancer treatment. Src, the first mammalian proto-oncogene to be discovered, is a valuable anti-cancer therapeutic target. Although several c-Src inhibitors have reached the clinical stage, drug resistance remains a challenge during treatment. Herein, a positive feedback loop between a previously uncharacterized long non-coding RNA (lncRNA), which the authors renamed lncRNA-inducing c-Src tumor-promoting function (LIST), and c-Src is uncovered. LIST directly binds to and regulates the Y530 phosphorylation activity of c-Src. As a c-Src agonist, LIST promotes tumor chemoresistance and progression in vitro and in vivo in multiple cancer types. c-Src can positively regulate LIST transcription by activating the NF-κB signaling pathway and then recruiting the P65 transcription factor to the LIST promoter. Interestingly, the LIST/c-Src interaction is associated with evolutionary new variations of c-Src. It is proposed that the human-specific LIST/c-Src axis renders an extra layer of control over c-Src activity.

Interactions between LIST truncation andc-Src.jpg

Fig1. Interactions between LIST truncation and c-Src.

The differential expression ofc-Src activity.jpg

Fig2. The differential expression of c-Src activity between the drug-sensitive and drug-resistant groups was assessed.

Case Study 2: Makio Higuchi, 2021

ATP-competitive inhibitors have been developed as promising anti-cancer agents. However, drug-resistance frequently occurs, and the underlying mechanisms are not fully understood. Here, we show that the activation of c-Src and its downstream phosphorylation cascade can be paradoxically induced by Src-targeted and RTK-targeted kinase inhibitors. We reveal that inhibitor binding induces a conformational change in c-Src, leading to the association of the active form c-Src with focal adhesion kinase (FAK). Reduction of the inhibitor concentration results in the dissociation of inhibitors from the c-Src-FAK complex, which allows c-Src to phosphorylate FAK and initiate FAK-Grb2-mediated Erk signaling. Furthermore, a drug-resistant mutation in c-Src, which reduces the affinity of inhibitors for c-Src, converts Src inhibitors into facilitators of cell proliferation by enhancing the phosphorylation of FAK and Erk in c-Src-mutated cells.

Translocation ofc-Src-EGFP.jpg

Fig3. Translocation of c-Src-EGFP to FAs induced by 30 nM dasatinib (top) in XTC cells and its dose dependency (bottom).

Western blot analysis of Src.jpg

Fig4. Western blot analysis of phospho-Y419 Src, Src (detected with a mouse monoclonal antibody), phospho-Erk (pErk), and Erk1&2 (Erk) in MCF-7 cells treated with inhibitors and subjected to inhibitor washout.

SRC involved in several pathways and played different roles in them. We selected most pathways SRC participated on our site, such as ErbB signaling pathway, Rap signaling pathway, Chemokine signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with SRC were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
ErbB signaling pathwayPLCG1;MAP2K2B;MAP2K7;PIK3CB;RPS6KB1B;PIK3R5;NCK1;EREG;HRAS
Rap signaling pathwayPIK3R5;FGFR4;MAP2K6;PRKD3;LPAR4;GRIN1;PLCB3;SIPA1L1;ITGA2B
Chemokine signaling pathwayCCR7;RAC1;PRKACG;Ccl21c;RAC2;FGR;CCL5;CCL15;GNGT2
EndocytosisWIPF2;PLD1;ARPC5L;PRKCI;PIP5K1B;CAV2;HSC70;WASLB;TGFBR1B
VEGF signaling pathwayPIK3CG;PIK3CA;PTGS2;HSPB1;MAPK14B;PLCG1;PIK3CB;PLA2G4D;KRAS
Focal adhesionBAD;VAV2;DOCK1;CAPN2A;RHOA;PAK7;MYL12A;COL4A6;VASP
Adherens junctionSNAI1;PTPRJ;FER;PARD3;ACTN4;LMO7;ACTN3A;CSNK2B;NLK2
Tight junctionRAB3B;SRC;MYH7B;CLDN7B;PTEN;PARD3;PPP2R2BB;PRKCD;CLDN7A
Gap junctionTUBA4A;PDGFRB;DRD1B;HTR2B;PDGFB;GNA15.1;TUBB5;PRKCB;TUBA1L
Platelet activationMYL12B;PIK3CA;FGB;VASP;PRKG1;RAP1B;PIK3CG;PIK3CD;COL24A1
GABAergic synapseGLS;SLC6A1;GABRP;GABBR1;GABRE;GABRA4;GNG10;GNG13;PRKACB
Inflammatory mediator regulation of TRP channelsPRKCH;ASIC5;MAP2K3;HTR2B;PTGER4;ITPR3;PLA2G4A;ITPR1;PIK3R1
Regulation of actin cytoskeletonINSRR;PPP1R12A;NRAS;MRAS;FGF14;MYL2;FGFR2;ACTB1;PTK2AA
GnRH signaling pathwayPLA2G4D;CALM1A;ADCY1B;HBEGFA;GNAS;GNRHR2;MAP3K3;MMP14;RAF1A
Estrogen signaling pathwaySP1;OPRM1;GPR30;KCNJ5;PIK3CG;CALML5;CALM1;CREB3L1;PRKCD
Prolactin signaling pathwayRELA;PIK3CG;INS2;PIK3CA;SLC2A2;PRLR;TNFSF11;SHC2;CCND1
Thyroid hormone signaling pathwayTHRA;TBC1D4;MAPK1;RXRG;AKT2;ITGB3;PRKCB;SRC;PIK3R2
Oxytocin signaling pathwayCALML3;ACTG1;PIK3CA;PLCB4;PPP3R1;CACNG8;MAPK1;CAMKK1;RHOA
Bacterial invasion of epithelial cellsARPC3;GAB1;ARPC1B;SHC2;SEPT6;SHC3;ARPC5;SEPT1;SEPT11
Epithelial cell signaling in Helicobacter pylori infectionJAM2;MAPK12;CCL5;NOD1;MAPK13;ATP6V0A4;F11R;SRC;PLCG1
ShigellosisU2AF1;IL-8;FBXW11;ACTB;MAPK11;ACTG1;ABL1;ITGB1;ITGA5
TuberculosisFCGR1;FCGR2A;MRC2;CALM1;RFXANK;RFXAP;IFNA12;PLK3;PPP3CB
Hepatitis BMAPK3;CYCS;CCNA1;ELK1;IFNA21;CCNE2;IL-8;IFNA13;TNF
Viral carcinogenesisSKP2;IL6ST;ATF4;HIST2H4A;HDAC10;NFKB2;HIST2H2BE;GTF2H2C;TRAF3
Proteoglycans in cancerVEGFA;FLNA;PTPN11;WNT7A;EZR;COL21A1;PLCG2;WNT16;SDC4
Bladder cancerBRAF;MAP2K2;DAPK2;E2F1;CDK4;SRC;RAF1;HRAS;DAPK1

SRC has several biochemical functions, for example, ATP binding, SH2 domain binding, SH3/SH2 adaptor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by SRC itself. We selected most functions SRC had, and list some proteins which have the same functions with SRC. You can find most of the proteins on our site.

Function Related Protein
ATP bindingPAPOLA;MARS;MAPKAPK2;NLRX1;NME2B.2;ADCY7;MYH1;NLRP4B;LIMK2
SH2 domain bindingARHGAP5;KHDRBS2;SH3PXD2B;TRPV4;SQSTM1;INPPL1;GNB2L1;AFAP1L2;LCK
SH3/SH2 adaptor activityRUSC1;CRKL;NCK2;SKAP1;KHDRBS1;SH2D2A;GRB14;LAT;FCRL2
enzyme bindingDNM2;ZNHIT6;UGT1A3;PDCD2;POLB;PTPLAD1;TULP3;HAND1;DDC
ephrin receptor bindingSDCBP;EFNA4;SRC;NTRK1;SIPA1L1;EFNA1;ANKS1B;EFNA5B;EFNB3B
growth factor receptor bindingAPP;SLC9A3R1;SRC;FYN
heme bindingCYP2D26;CYP2C38;CYP2F1;CYGB1;CYP3C1;MPX;SRC;PTGES2;CYP1C1
hormone receptor bindingJAK2A;JAK2B;JAK3;TNK2;TNK2B;SRC;TNK1
integrin bindingTHY1;ITGA6;CYR61L1;CD226;CD151;GPNMB;KDR;ANXA7;NIPAL1
ion channel bindingTRPC1;PACS1A;AP2M1;KCNE2;CAMK2D;PKD2;RNF207;ACTN1;RIMS1
kinase activityPIK3R3B;PDK2B;PAK2A;TLK1B;Aatk;KITA;PKN1B;IGF1RA;BCKDK
kinase bindingTNFAIP3;BTG1;PARK7;CAB39;SNAI1;LDHA;JAKMIP1;DLG3;RASGRP3
non-membrane spanning protein tyrosine kinase activityPTK2BB;JAK1;BLK;ABL2;PTK2B;BTK;FER;RIPK2;FGR
phosphoprotein bindingTBK1;APTX;DPYS;SERPINB1A;MID1;MTOR;MS4A2;SRC;IGF2R
protein bindingOCA2;RNF14;SCEL;MCM8;ABCG2;FAM134B;PSMB1;PLEKHG2;FAM9A
protein kinase activityKALRN;MAPK8A;RNASEL;DCKL1B;LTK;PPP1R9B;PTK6B;BRAF;CHUK
protein tyrosine kinase activityEPHA2A;TYK2;FYN;JAK2;ERBB4A;DYRK4;MUSK;JAK2B;CLK4
receptor bindingCADM1A;FGF24;SCYL2;INSL4;FGF10B;SNX17;VTCN1;LBP;INHA
scaffold protein bindingCASP8;NCK2;HOMER1;KCNA5;LRP4;P2RY1;NOS3;ATP2B4;NOS1

SRC has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SRC here. Most of them are supplied by our site. Hope this information will be useful for your research of SRC.

PTPN1; PTK2

Gene Family

GPCRs TGF

Research Area

Related articles

Liu, Y; Zhang, LW; et al. DNA-PKcs Deficiency Inhibits Glioblastoma Cell-Derived Angiogenesis After Ionizing Radiation. JOURNAL OF CELLULAR PHYSIOLOGY 230:1094-1103(2015).
He, YX; Liu, J; et al. Src inhibitor reduces permeability without disturbing vascularization and prevents bone destruction in steroid-associated osteonecrotic lesions in rabbits. SCIENTIFIC REPORTS 5:-(2015).
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Q&As (7)

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How do alterations in SRC expression or activity affect the development of drug resistance in tumors? 10/23/2022

Changes in SRC expression or kinase activity can lead to drug resistance in cancer treatment.

How does SRC (proto-oncogene tyrosine-protein kinase Src) regulate cell proliferation and survival? 07/08/2021

SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.

How does SRC contribute to the regulation of bone remodeling and osteoclast activity? 06/26/2020

SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.

What role does SRC play in cancer development and progression, particularly in metastasis? 03/16/2020

SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.

How does SRC influence cellular signaling pathways, such as the Ras/MAPK and PI3K/Akt pathways? 12/06/2019

SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.

What are the mechanisms by which SRC modulates cellular adhesion and migration? 03/27/2019

SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.

What is the impact of SRC inhibition on targeted cancer therapies? 01/02/2018

SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.

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    Top-notch protein purification service, essential for our research.

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      Reliable mass spectrometry analysis, pivotal for our proteomics work.

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        Swift and reliable protein sequencing, indispensable for our research success.

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