| Description : |
ULK3 (Unc-51 Like Kinase 3) is a Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. It acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). It also positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. ULK3 Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
1.368mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution. |
| Bio-activity : |
302U/mg, where one unit of ULK3, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
62 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GRRMRAAFPA GGAGGSVEPP SARPAPQPAG 61 TAARSEEAPA RAQAAGMAGP GWGPPRLDGF ILTERLGSGT YATVYKAYAK KDTREVVAIK 121 CVAKKSLNKA SVENLLTEIE ILKGIRHPHI VQLKDFQWDS DNIYLIMEFC AGGDLSRFIH 181 TRRILPEKVA RVFMQQLASA LQFLHERNIS HLDLKPQNIL LSSLEKPHLK LADFGFAQHM 241 SPWDEKHVLR GSPLYMAPEM VCQRQYDARV DLWSMGVILY EALFGQPPFA SRSFSELEEK 301 IRSNRVIELP LRPLLSRDCR DLLQRLLERD PSRRISFQDF FAHPWVDLEH MPSGESLGRA 361 TALVVQAVKK DQEGDSAAAL SLYCKALDFF VPALHYEVDA QRKEAIKAKV GQYVSRAEEL 421 KAIVSSSNQA LLRQGTSARD LLREMARDKP RLLAALEVAS AAMAKEEAAG GEQDALDLYQ 481 HSLGELLLLL AAEPPGRRRE LLHTEVQNLM ARAEYLKEQV KMRESRWEAD TLDKEGLSES 541 VRSSCTLQ |
| Purity : |
Purity 91.7% by SDS-PAGE and Coomassie staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
