Recombinant Mouse Ulk3 Protein, Myc/DDK-tagged

• Cat.No.: Ulk3-6831M
• Product Overview: Purified recombinant protein of mouse full-length unc-51-like kinase 3 (Ulk3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 53.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ulk3 unc-51-like kinase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Ulk3
• Synonyms: ULK3; unc-51-like kinase 3 (C. elegans); serine/threonine-protein kinase ULK3; 1200015E14Rik
• Gene ID: 71742
• mRNA Refseq: NM_027895
• Protein Refseq: NP_082171
• UniProt ID: Q3U3Q1