Active Recombinant Full Length Human ZAP70 Protein, C-His-tagged

• Cat.No.: ZAP70-187HFL
• Product Overview: C-terminal 6His-tagged, recombinant, human, full-length ZAP-70, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: ZA70 (Tyrosine Protein kinase ZAP-70, 70 kDa zeta-associated protein, Syk-related tyrosine kinase) is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. ZA70 is expressed in human and murine thymocyte subsets, B cells, and peripheral T cell subsets and associates with zeta-chain to undergo tyrosine phosphorylation following TCR stimulation. ZA70 inhibitors may be useful as immunosuppressants.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.134mg/ml of enzyme in of 25mMHepes/NaOH pH7.4, 150mM NaCl, 0.5mM DTT, 50% glycerol. Liquid at -20 centigrade.
• Bio-activity: 62U/mg, where one unit of ZAP-70 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 70.8 kDa
• AA Sequence: 1 MPDPAAHLPF FYGSISRAEA EEHLKLAGMA DGLFLLRQCL RSLGGYVLSL VHDVRFHHFP 61 IERQLNGTYA IAGGKAHCGP AELCEFYSRD PDGLPCNLRK PCNRPSGLEP QPGVFDCLRD 121 AMVRDYVRQT WKLEGEALEQ AIISQAPQVE KLIATTAHER MPWYHSSLTR EEAERKLYSG 181 AQTDGKFLLR PRKEQGTYAL SLIYGKTVYH YLISQDKAGK YCIPEGTKFD TLWQLVEYLK 241 LKADGLIYCL KEACPNSSAS NASGAAAPTL PAHPSTLTHP QRRIDTLNSD GYTPEPARIT 301 SPDKPRPMPM DTSVYESPYS DPEELKDKKL FLKRDNLLIA DIELGCGNFG SVRQGVYRMR 361 KKQIDVAIKV LKQGTEKADT EEMMREAQIM HQLDNPYIVR LIGVCQAEAL MLVMEMAGGG 421 PLHKFLVGKR EEIPVSNVAE LLHQVSMGMK YLEEKNFVHR DLAARNVLLV NRHYAKISDF 481 GLSKALGADD SYYTARSAGK WPLKWYAPEC INFRKFSSRS DVWSYGVTMW EALSYGQKPY 541 KKMKGPEVMA FIEQGKRMEC PPECPPELYA LMSDCWIYKW EDRPDFLTVE QRMRACYYSL 601 ASKVEGPPGS TQKAEAACAH HHHHH
• Purity: Purity 90.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ZAP70 zeta chain of T cell receptor associated protein kinase 70 [ Homo sapiens (human) ]
• Official Symbol: ZAP70
• Synonyms: ZAP70; zeta chain of T cell receptor associated protein kinase 70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Gene ID: 7535
• mRNA Refseq: NM_001079.4
• Protein Refseq: NP_001070.2
• MIM: 176947
• UniProt ID: P43403