Recombinant Full Length Human ZAP70 Protein, His tagged

• Cat.No.: ZAP70-98H
• Product Overview: Recombinant human full-length protein produced from recombinant baculovirus in insect cells, Histidine-tagged. No special measures were taken to activate this kinase.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-619 aa
• Description: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
• Form: 25 mM HEPES (pH 7.5), 150 mM NaCl, 0.5 mM DTT, 50% Glycerol.
• Bio-activity: 410 nmoles of phosphate transferred to poly [Glu, Tyr] 4:1 per minute per mg of total protein at 37 centigrade. Activity determined at a final protein concentration of 1 μg/mL.
• Molecular Mass: 70.1 kDa
• Purity: 90% as determined by a SDS-PAGE gel stained with SimplyBlue SafeStain.
• Storage: Store at -20 centigrade. At first use, aliquot and store at -20 centigrade to avoid multiple freeze-thaws. If properly stored at -20 centigrade, this product is guaranteed for 6 months from date of purchase.
• Concentration: 0.47 mg/mL total protein as measured using the Bradford protein assay with BSA as a standard.

Gene Information

• Gene Name: ZAP70 zeta chain of T cell receptor associated protein kinase 70 [ Homo sapiens (human) ]
• Official Symbol: ZAP70
• Synonyms: ZAP70; zeta chain of T cell receptor associated protein kinase 70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70; tyrosine-protein kinase ZAP-70; ; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; syk-related tyrosine kinase; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD; EC 2.7.10.2
• Gene ID: 7535
• mRNA Refseq: NM_001079
• Protein Refseq: NP_001070
• MIM: 176947
• UniProt ID: P43403