Active Recombinant Full Length Mouse PIK3CB and PIK3R1 Co-expressed Protein, N-His-tagged

• Cat.No.: PIK3CB-PIK3R1-141MFL
• Product Overview: Complex of N-terminal 6His-tagged, recombinant mouse p110β, full length and untagged, recombinant, mouse p85α, full length. Co-expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: PI3 Kinase plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. It also participates in cellular signaling in response to various growth factors and involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. PI3K regulates invadopodia formation through the PDPK1-AKT1 pathway, and participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: Full Length
• Form: 0.893mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 508U/mg, where one unit of PI 3-Kinase (p110β/p85α) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate (PIP3) formed per minute at room temperature with a final ATP concentration of 100μM.
• Molecular Mass: p110β MW = 126kDa, p85α MW = 84kDa
• AA Sequence: p110β amino acid sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MPPAMADNLD IWAVDSQIAS DGAISVDFLL 61 PTGIYIQLEV PREATISYIK QMLWKQVHNY PMFNLLMDID SYMFACVNQT AVYEELEDET 121 RRLCDVRPFL PVLKLVTRSC DPAEKLDSKI GVLIGKGLHE FDALKDPEVN EFRRKMRKFS 181 EAKIQSLVGL SWIDWLKHTY PPEHEPSVLE NLEDKLYGGK LVVAVHFENS QDVFSFQVSP 241 NLNPIKINEL AIQKRLTIRG KEDEASPCDY VLQVSGRVEY VFGDHPLIQF QYIRNCVMNR 301 TLPHFILVEC CKIKKMYEQE MIAIEAAINR NSSNLPLPLP PKKTRVISHI WDNNNPFQIT 361 LVKGNKLNTE ETVKVHVRAG LFHGTELLCK TVVSSEISGK NDHIWNEQLE FDINICDLPR 421 MARLCFAVYA VLDKVKTKKS TKTINPSKYQ TIRKAGKVHY PVAWVNTMVF DFKGQLRSGD 481 VILHSWSSFP DELEEMLNPM GTVQTNPYAE NATALHITFP ENKKQPCYYP PFDKIIEKAA 541 ELASGDSANV SSRGGKKFLA VLKEILDRDP LSQLCENEMD LIWTLRQDCR ENFPQSLPKL 601 LLSIKWNKLE DVAQLQALLQ IWPKLPPREA LELLDFNYPD QYVREYAVGC LRQMSDEELS 661 QYLLQLVQVL KYEPFLDCAL SRFLLERALD NRRIGQFLFW HLRSEVHTPA VSVQFGVILE 721 AYCRGSVGHM KVLSKQVEAL NKLKTLNSLI KLNAVKLSRA KGKEAMHTCL KQSAYREALS 781 DLQSPLNPCV ILSELYVEKC KYMDSKMKPL WLVYSSRAFG EDSVGVIFKN GDDLRQDMLT 841 LQMLRLMDLL WKEAGLDLRM LPYGCLATGD RSGLIEVVST SETIADIQLN SSNVAATAAF 901 NKDALLNWLK EYNSGDDLDR AIEEFTLSCA GYCVASYVLG IGDRHSDNIM VKKTGQLFHI 961 DFGHILGNFK SKFGIKRERV PFILTYDFIH VIQQGKTGNT EKFGRFRQCC EDAYLILRRH 1021 GNLFITLFAL MLTAGLPELT SVKDIQYLKD SLALGKSEEE ALKQFKQKFD EALRESWTTK 1081 VNWMAHTVRK DYRS
  p85α amino acid sequence:
  1 MSAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EDIGWLNGYN
  61 ETTGERGDFP GTYVEYIGRK RISPPTPKPR PPRPLPVAPG SSKTEADTEQ QALPLPDLAE
  121 QFAPPDVAPP LLIKLLEAIE KKGLECSTLY RTQSSSNPAE LRQLLDCDAA SVDLEMIDVH
  181 VLADAFKRYL ADLPNPVIPV AVYNEMMSLA QELQSPEDCI QLLKKLIRLP NIPHQCWLTL
  241 QYLLKHFFKL SQASSKNLLN ARVLSEIFSP VLFRFPAASS DNTEHLIKAI EILISTEWNE
  301 RQPAPALPPK PPKPTTVANN SMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA
  361 STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFNS VVELINHYRN ESLAQYNPKL
  421 DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK
  481 RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNHDK LKSRISEIID
  541 SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN
  601 ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS
  661 VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA
  721 QQRR
• Purity: Purity (p110β and p85α combined) 80.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Pik3cb phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta [ Mus musculus (house mouse) ] and Pik3r1 phosphoinositide-3-kinase regulatory subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: PIK3CB and PIK3R1
• Synonyms: PIK3CB and PIK3R1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta and phosphoinositide-3-kinase regulatory subunit 1; p110beta; 1110001J02Rik and PI3K; p50alpha; p55alpha; p85alpha
• Gene ID: 74769 and 18708
• mRNA Refseq: NM_029094.3 and NM_001024955.2
• Protein Refseq: NP_083370.2 and NP_001020126.1
• UniProt ID: Q8BTI9 and P26450