Active Recombinant HIV-1 YU2 (M Tropic) gp120 Envelope Glycoprotein

• Cat.No.: HIV1YU2gp120-107H

Specification

• Description: Gene cloned from Macrophage -cell-tropic HIV-1 YU2 strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System. This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
• Source: Insect cells
• Species: HIV
• Form: Frozen Liquid
• Bio-activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti gp120 mAb-peroxidase capture ELISA. Cell activation assays in progress.
• Purity: > 95 % by SDS-PAGE
• Applications: CD4 Binding;
  Cell Activation;
  In-Vitro Diagnostics.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
  ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
• Stability: At least 6 months at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: PBS