Active Recombinant Human AXL(35-end) Protein, N-His-tagged

• Cat.No.: AXL-27H
• Product Overview: N-terminal 5His-tagged, recombinant amino acids 473–end human Axl expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: AXL is a member of AXL protein-tyrosine kinase family and expressed human myeloid leukemia cells. Gas6, the protein product of the growth arrest-specific gene 6 (gas6), a member of the vitamin K-dependent protein family, was identified as a ligand for the Axl/Sky family of receptor tyrosine kinases. Gas6/Axl signaling has been related to the cell growth and protection from apoptosis in normal and cancer cells.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 35-end
• Form: 1.158mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 142U/mg, where one unit of Axl, active activity is defined as 1nmol phosphate incorporated into 250μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48 kDa
• AA Sequence: 1 MHHHHHHRRK KETRYGEVFE PTVERGELVV RYRVRKSYSR RTTEATLNSL GISEELKEKL 61 RDVMVDRHKV ALGKTLGEGE FGAVMEGQLN QDDSILKVAV KTMKIAICTR SELEDFLSEA 121 VCMKEFDHPN VMRLIGVCFQ GSERESFPAP VVILPFMKHG DLHSFLLYSR LGDQPVYLPT 181 QMLVKFMADI ASGMEYLSTK RFIHRDLAAR NCMLNENMSV CVADFGLSKK IYNGDYYRQG 241 RIAKMPVKWI AIESLADRVY TSKSDVWSFG VTMWEIATRG QTPYPGVENS EIYDYLRRGN 301 RLKQPADCLD GLYALMSRCW ELNPQDRPSF TELREDLENT LKALPPAQEP DEILYVNMDE 361 GGGYPEPPGA AGGADPPTQP DPKDSCSCLT AAEVHPAGRY VLCPSTTPSP AQPADRGSPA 421 APGQEDGA
• Purity: Purity 84% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AXL AXL receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: AXL
• Synonyms: AXL; AXL receptor tyrosine kinase; ARK; UFO; AXL3; JTK11; Tyro7
• Gene ID: 558
• mRNA Refseq: NM_001699.6
• Protein Refseq: NP_001690.2
• MIM: 109135
• UniProt ID: P30530