Recombinant Human AXL protein(Met1-Pro449), His-tagged

• Cat.No.: AXL-769H
• Product Overview: Recombinant Human AXL isoform 1 (AAB20305.1) extracellular domain (Met1-Pro449) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Pro449
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human AXL consists of 428 amino acids after removal of the signal peptide and predicts a molecular mass of 46.5 kDa. In SDS-PAGE under reducing conditions, it migRates as an approximately 60-70 kDa band due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AXL AXL receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: AXL
• Synonyms: AXL; AXL receptor tyrosine kinase; tyrosine-protein kinase receptor UFO; JTK11; UFO; AXL oncogene; oncogene AXL; AXL transforming sequence/gene
• Gene ID: 558
• mRNA Refseq: NM_001699
• Protein Refseq: NP_001690
• MIM: 109135
• UniProt ID: P30530

Citation

Harnessing MerTK agonism for targeted therapeutics

Journal: mAbs    PubMed ID: 31852344    Data: 2022/12/14

Authors: Vivekananda Kedage, Diego Ellerman, Minhong Yan

Article Snippet:ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.. Briefly, 1ug/ml of human MerTK (Genentech), human AXL (Creative BioMart, Catalog AXL-769H), human TYRO3 (Creative BioMart, Catalog TYRO3-195H), mouse MerTK (R&D systems, Catalog 591-MR-100), mouse AXL (Creative BioMart, Catalog AXL-626M), mouse TYRO3 (R&D systems, Catalog 759-DT-100) were coated onto ELISA plates.. The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.

Publication :

Efficacy of newly discovered DNA aptamers targeting AXL in a lung cancer cell with acquired resistance to Erlotinib (2021)

Harnessing MerTK agonism for targeted therapeutics (2020)

Conversion of RNA Aptamer into Modified DNA Aptamers Provides for Prolonged Stability and Enhanced Antitumor Activity (2021)