Active Recombinant Human CAMK2B(1-315) Protein, N-His-tagged

• Cat.No.: CAMK2B-38H
• Product Overview: N-terminal 6Histagged, recombinant, CaM Kinase IIβ, amino acids 1–315, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: CAMK2B (Calcium/Calmodulin Dependent Protein Kinase II Beta) belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-315 aa
• Form: 3.6mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 14744U/mg, where one unit of CaM Kinase IIβ activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39.6 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MATTVTCTRF TDEYQLYEDI GKGAFSVVRR 61 CVKLCTGHEY AAKIINTKKL SARDHQKLER EARICRLLKH SNIVRLHDSI SEEGFHYLVF 121 DLVTGGELFE DIVAREYYSE ADASHCIQQI LEAVLHCHQM GVVHRDLKPE NLLLASKCKG 181 AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKEA YGKPVDIWAC GVILYILLVG 241 YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN QMLTINPAKR ITAHEALKHP 301 WVCQRSTVAS MMHRQETVEC LKKFNARRKL KGAILTTMLA TRNFS
• Purity: Purity 86% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CAMK2B calcium/calmodulin dependent protein kinase II beta [ Homo sapiens (human) ]
• Official Symbol: CAMK2B
• Synonyms: CAMK2B; calcium/calmodulin dependent protein kinase II beta; CAM2; CAMK2; CAMKB; MRD54; CaMKIIbeta
• Gene ID: 816
• mRNA Refseq: NM_001220.5
• Protein Refseq: NP_001211.3
• MIM: 607707
• UniProt ID: Q13554