Active Recombinant Human CDKL1(1-303) Protein, N-His-tagged

• Cat.No.: CDKL1-44H
• Product Overview: N-terminal 6His-tagged, recombinant, human CDKL1 amino acids 1-303 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CDKL1 is a member of a large family of CDC2-related serine/threonine protein kinases that accumulates primarily in the nucleus. Diseases associated with CDKL1 include Gene Duplication Disease. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDKL4.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-303 aa
• Form: 1.36mg/ml of enzyme in 50mM Tris/HCl pH7.5, 280mM NaCl, 260mM imidazole, 0.09mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.9mM benzamidine, 0.2mM PMSF, 0.09% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 13U/mg, where one unit of CDKL1 activity is defined as 1nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SMMEKYEKIG KIGEGSYGVV FKCRNRDTGQ 61 IVAIKKFLES EDDPVIKKIA LREIRMLKQL KHPNLVNLLE VFRRKRRLHL VFEYCDHTVL 121 HELDRYQRGV PEHLVKSITW QTLQAVNFCH KHNCIHRDVK PENILITKHS VIKLCDFGFA 181 RLLTGPSDYY TDYVATRWYR SPELLVGDTQ YGPPVDVWAI GCVFAELLSG VPLWPGKSDV 241 DQLYLIRKTL GDLIPRHQQV FSTNQYFSGV KIPDPEDMEP LELKFPNISY PALGLLKGCL 301 HMDPTQRLTC EQLLHHPYFE NIREIEDLAK EHNK
• Purity: Purity 92% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDKL1 cyclin dependent kinase like 1 [ Homo sapiens (human) ]
• Official Symbol: CDKL1
• Synonyms: CDKL1; cyclin dependent kinase like 1; P42; KKIALRE
• Gene ID: 8814
• mRNA Refseq: NM_001282236.3
• Protein Refseq: NP_001269165.2
• MIM: 603441
• UniProt ID: Q00532