Active Recombinant Human CHEK2(5-543) Protein, N-GST,C-His-tagged

• Cat.No.: CHEK2-49H
• Product Overview: Recombinant human CHK2, residues 5–end, containing N-terminal GST and C-terminal 6His-tags. Expressed in E. coli cells and purified using glutathione-agarose and Ni2+/NTA-agarose.

Specification

• Description: Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK2 might have a redundant and supportive role in checkpoints, but its role in ionizing radiation-induced apoptosis is more prominent. However, its role in other DNA-damage-induced apoptosis mechanisms is less well established. CHK2 inhibitors are expected to sensitize proliferating cells to chemotherapy-induced apoptosis.
• Source: E. coli
• Species: Human
• Tag: GST,His
• Protein length: 5-543 aa
• Form: 1.004mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 1595U/mg, where one unit of CHK2, active activity is defined as 1nmol phosphate incorporated into 200μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 89.6 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSRRAS VGSHMPMSRP 241 RRPSDVEAQQ SHGSSACSQP HGSVTQSQGS SSQSQGISSS STSTMPNSSQ SSHSSSGTLS 301 SLETVSTQEL YSIPEDQEPE DQEPEEPTPA PWARLWALQD GFANLECVND NYWFGRDKSC 361 EYCFDEPLLK RTDKYRTYSK KHFRIFREVG PKNSYIAYIE DHSGNGTFVN TELVGKGKRR 421 PLNNNSEIAL SLSRNKVFVF FDLTVDDQSV YPKALRDEYI MSKTLGSGAC GEVKLAFERK 481 TCKKVAIKII SKRKFAIGSA READPALNVE TEIEILKKLN HPCIIKIKNF FDAEDYYIVL 541 ELMEGGELFD KVVGNKRLKE ATCKLYFYQM LLAVQYLHEN GIIHRDLKPE NVLLSSQEED 601 CLIKITDFGH SKILGETSLM RTLCGTPTYL APEVLVSVGT AGYNRAVDCW SLGVILFICL 661 SGYPPFSEHR TQVSLKDQIT SGKYNFIPEV WAEVSEKALD LVKKLLVVDP KARFTTEEAL 721 RHPWLQDEDM KRKFQDLLSE ENESTALPQV LAQPSTSRKR PREGEAEGAE TTKRPAVCAA 781 VLHHHHHH
• Purity: Purity 69.3% by SDSPAGE and Coomassie staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CHEK2 checkpoint kinase 2 [ Homo sapiens (human) ]
• Official Symbol: CHEK2
• Synonyms: CHEK2; checkpoint kinase 2; CDS1; CHK2; LFS2; RAD53; TPDS4; hCds1; HuCds1; PP1425
• Gene ID: 11200
• mRNA Refseq: NM_001005735.2
• Protein Refseq: NP_001005735.1
• MIM: 604373
• UniProt ID: O96017