Recombinant Human CHEK2 Protein, GST-Tagged

• Cat.No.: CHEK2-1223H
• Product Overview: Human CHEK2 full-length ORF (AAH04207.1, 1 a.a. - 543 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 85.14 kDa
• AA Sequence: MSRESDVEAQQSHGSSACSQPHGSV TQSQGSSSQSQGISSSSTSTMPNSS QSSHSSSGTLSSLETVSTQELYSIP EDQEPEDQEPEEPTPAPWARLWALQ DGFANLECVNDNYWFGRDKSCEYCF DEPLLKRTDKYRTYSKKHFRIFREV GPKNSYIAYIEDHSGNGTFVNTELV GKGKRRPLNNNSEIALSLSRNKVFV FFDLTVDDQSVYPKALRDEYIMSKT LGSGACGEVKLAFERKTCKKVAIKI ISKRKFAIGSAREADPALNVETEIE ILKKLNHPCIIKIKNFFDAEDYYIV LELMEGGELFDKVVGNKRLKEATCK LYFYQMLLAVQYLHENGIIHRDLKP ENVLLSSQEEDCLIKITDFGHSKIL GETSLMRTLCGTPTYLAPEVLVSVG TAGYNRAVDCWSLGVILFICLSGYP PFSEHRTQVSLKDQITSGKYNFIPE VWAEVSEKALDLVKKLLVVDPKARF TTEEALRHPWLQDEDMKRKFQDLLS EENESTALPQVLAQPSTSRKRPREG EAEGAETTKRPAVCAAVL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CHEK2 checkpoint kinase 2 [ Homo sapiens ]
• Official Symbol: CHEK2
• Synonyms: CHEK2; checkpoint kinase 2; CHK2 (checkpoint, S.pombe) homolog, CHK2 checkpoint homolog (S. pombe), RAD53; serine/threonine-protein kinase Chk2; bA444G7; CDS1; CHK2; HuCds1; PP1425; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2; LFS2; RAD53; hCds1
• Gene ID: 11200
• mRNA Refseq: NM_001005735
• Protein Refseq: NP_001005735
• MIM: 604373
• UniProt ID: O96017