| Description : |
Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK2 might have a redundant and supportive role in checkpoints, but its role in ionizing radiation-induced apoptosis is more prominent. However, its role in other DNA-damage-induced apoptosis mechanisms is less well established. CHK2 inhibitors are expected to sensitize proliferating cells to chemotherapy-induced apoptosis. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST,His |
| Protein length : |
5-end |
| Form : |
0.262mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
143U/mg, where one unit of CHK2 (R145W), active activity is defined as 1nmol phosphate incorporated into 100μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
89.6 kDa |
| AA Sequence : |
1 MSPISRMPIL GYWKIKGLVQ PTRLLLEYLE EKYEEHLYER DEGDKWRNKK FELGLEFPNL 61 PYYIDGDVKL TQSMAIIRYI ADKHNMLGGC PKERAEISML EGAVLDIRYG VSRIAYSKDF 121 ETLKVDFLSK LPEMLKMFED RLCHKTYLNG DHVTHPDFML YDALDVVLYM DPMCLDAFPK 181 LVCFKKRIEA IPQIDKYLKS SKYIAWPLQG WQATFGGGDH PPKSDLVPRG SRRASVGSHM 241 PMSRPRRPSD VEAQQSHGSS ACSQPHGSVT QSQGSSSQSQ GISSSSTSTM PNSSQSSHSS 301 SGTLSSLETV STQELYSIPE DQEPEDQEPE EPTPAPWARL WALQDGFANL ECVNDNYWFG 361 RDKSCEYCFD EPLLKRTDKY RTYSKKHFWI FREVGPKNSY IAYIEDHSGN GTFVNTELVG 421 KGKRRPLNNN SEIALSLSRN KVFVFFDLTV DDQSVYPKAL RDEYIMSKTL GSGACGEVKL 481 AFERKTCKKV AIKIISKRKF AIGSAREADP ALNVETEIEI LKKLNHPCII KIKNFFDAED 541 YYIVLELMEG GELFDKVVGN KRLKEATCKL YFYQMLLAVQ YLHENGIIHR DLKPENVLLS 601 SQEEDCLIKI TDFGHSKILG ETSLMRTLCG TPTYLAPEVL VSVGTAGYNR AVDCWSLGVI 661 LFICLSGYPP FSEHRTQVSL KDQITSGKYN FIPEVWAEVS EKALDLVKKL LVVDPKARFT 721 TEEALRHPWL QDEDMKRKFQ DLLSEENEST ALPQVLAQPS TSRKRPREGE AEGAETTKRP 781 AVCAAVLHHH HHH |
| Purity : |
Purity 62% by SDS-PAGE and Coomassie staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| References : |
Wu X et al, JBC (2001);276:2971-2974 and Lee SB et al, Cancer Research (2001);61:8062-8067 |
