| Description : |
Mammalian casein kinase 1 (CK1) gene family consists of seven different genes in mammals, CKIa, b, g1, g2, g3, d and e. These enzymes are important component of signal transduction pathways, regulates cell growth, division and differentiation, and the activities of a variety of oncoproteins and transcription factors. CSNK1G1 (Casein kinase I gamma1 isoform) plays an important role in cell survival and protects regulatory proteins from caspase-mediated degradation in apoptosis. Inhibitors may find clinical use for the treatment of Alzheimer's disease and amyotrophic lateral sclerosis. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
1-330 aa |
| Form : |
2.367mg/ml of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
19751U/mg, where one unit of Casein Kinase 1γ1 activity is defined as 1nmol phosphate incorporated into 200μM (KRRRALS(p)VASLPGL) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
41.9 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF CSRPSGSSSS SGVLMVGPNF RVGKKIGCGN 61 FGELRLGKNL YTNEYVAIKL EPIKSRAPQL HLEYRFYKQL GSAGEGLPQV YYFGPCGKYN 121 AMVLELLGPS LEDLFDLCDR TFTLKTVLMI AIQLLSRMEY VHSKNLIYRD VKPENFLIGR 181 QGNKKEHVIH IIDFGLAKEY IDPETKKHIP YREHKSLTGT ARYMSINTHL GKEQSRRDDL 241 EALGHMFMYF LRGSLPWQGL KADTLKERYQ KIGDTKRNTP IEALCENFPE EMATYLRYVR 301 RLDFFEKPDY EYLRTLFTDL FEKKGYTFDY AYDWVGRPIP TPVGSVHVDS GASAITRESH 361 T |
| Purity : |
Purity 93% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
