Active Recombinant Human DAPK1(1-296) Protein, N-GST-tagged

• Cat.No.: DAPK1-52H
• Product Overview: N-terminal GSTtagged, recombinant, human DAPK1, amino acids 1–296, expressed by baculovirus in Sf21 insect cells. Purified using glutathioneagarose.

Specification

• Description: Death-associated protein kinase 1 (DAPK1) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK1 modulates cell death induced by IFN-γ, TNF-α, Fas, and detachment from the extracellular matrix. DAPK1 has been also implicated in TGF-β-induced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK1.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-296 aa
• Form: 2.3mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 879U/mg, where one unit of DAPK1 activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM
• Molecular Mass: 60.9 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MTVFRQENVD 241 DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK SSRRGVSRED IEREVSILKE 301 IQHPNVITLH EVYENKTDVI LILELVAGGE LFDFLAEKES LTEEEATEFL KQILNGVYYL 361 HSLQIAHFDL KPENIMLLDR NVPKPRIKII DFGLAHKIDF GNEFKNIFGT PEFVAPEIVN 421 YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY FSNTSALAKD 481 FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKF
• Purity: Purity 97% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DAPK1 death associated protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death associated protein kinase 1; DAPK; ROCO3
• Gene ID: 1612
• mRNA Refseq: NM_001288729.2
• Protein Refseq: NP_001275658.1
• MIM: 600831
• UniProt ID: P53355