Active Recombinant Human DAPK3(1-290) Protein, N-GST-tagged

• Cat.No.: DAPK3-261H
• Product Overview: N-terminal, GST tagged, recombinant, of human ZIPK amino acids 1–290, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: ZIP (or DAPK3 : Death-associated protein kinase) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. It has been shown to have a potential role in regulating VSMC contraction and induces morphological changes in apoptosis when overexpressed in mammalian cells.
• Source: E. coli
• Species: Human
• Tag: His
• Protein length: 1-290 aa
• Form: 0.361mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 239U/mg, where one unit of ZIPK, active activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 64.3 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFG TRGGVAIRGL 241 LRSYLQAAVT ALSLEWKLLE GGPAAMSTFR QEDVEDHYEM GEELGSGQFA IVRKCRQKGT 301 GKEYAAKFIK KRRLSSSRRG VSREEIEREV NILREIRHPN IITLHDIFEN KTDVVLILEL 361 VSGGELFDFL AEKESLTEDE ATQFLKQILD GVHYLHSKRI AHFDLKPENI MLLDKNVPNP 421 RIKLIDFGIA HKIEAGNEFK NIFGTPEFVA PEIVNYEPLG LEADMWSIGV ITYILLSGAS 481 PFLGETKQET LTNISAVNYD FDEEYFSNTS ELAKDFIRRL LVKDPKRRMT IAQSLEHSWI 541 KAIRRRNVRG EDSGRIVTD
• Purity: Purity 56.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DAPK3 death associated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: DAPK3
• Synonyms: DAPK3; death associated protein kinase 3; DLK; ZIP; ZIPK
• Gene ID: 1613
• mRNA Refseq: NM_001348.3
• Protein Refseq: NP_001339.1
• MIM: 603289
• UniProt ID: O43293